RP-HPLC法测定小儿清热片中大黄5种蒽醌类成分含量  

Determination of five anthraquinones in Rheum officinale from Xiao'er Qingre Tablets by RP-HPLC

作　　者：余积聪 李俊健[1] 吴永成[1] 宋粉云[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《广东药学院学报》2013年第5期510-513,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金(5002841)

摘　　要：目的建立测定小儿清热片中大黄5种蒽醌类成分含量的方法。方法采用反相高效液相色谱法，固定相为Diamonsil—C18柱，甲醇-0．2％（体积分数）H，PO。水溶液（体积比86．5：13．5）为流动相，流速为1．0mL·min^-1，检测波长为430nm，柱温为30℃。结果大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素质量浓度分别在2．0—12．0、3．6～21．6、16．0—96．0、5．4～32．4、3．0—18．0μg·mL^-1范同内具有良好的线性关系，平均回收率分别为102．1％、100．5％、103．1％、100．7％和99．3％（I=6）。结论该法可用于小儿清热片中大黄5种蒽醌类成分的含量测定。Objective To establish a method for the assay of five anthraquinones in Rheum officinale from Xiao＇er Qingre Tablets. Methods An HPLC method was established. The chromatographic column was Diamonsil-C18. The mobile phase was methanol-0. 2% phosphoric acid（86.5： 13.5）. The flow rate was 1.0 mL . min-1 , and the detection wavelength was 430 nm. The column temperature was 30 ℃. Results The calibration curves for emodin, rhein, chrysophanol, physcion and aloe-emodin were linear in the range of 2.0-12.0,3.6-21.6,16.0-96.0,5.4-32.4,3.0- 18.0 μg . mL^-1. The average recovery for emodin,rhein, chrysophanol, physcion and aloe-emodin were 102. 1% , 100. 5% , 103.1% , 100. 7% and 99. 3% （n = 6）, respectively. Conclusion The method can be used for quantitative determination of five anthraquinones in Rheum officinale from Xiao＇er Qingre Tablets.

关 键 词：小儿清热片 大黄 蒽醌类成分 反相高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学]