蚓激酶抗角叉菜胶诱导大鼠血栓形成作用  

Effect of Lumbrokinase on Thrombosis Induced by Carrageenan in Rats

作　　者：谢德芳[1] 方成[1] 王春维[2] 周海[2] 

机构地区：[1]武汉工业学院生物与制药工程学院,武汉430023 [2]武汉工业学院食品科学与工程学院,武汉430023

出　　处：《中国医药导刊》2013年第11期1877-1878,1880,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:通过大鼠角叉菜胶诱导大鼠血栓模型,对从赤子爱胜蚓中提取的蚓激酶的溶栓效果进行研究。方法:大鼠随机分为正常组、模型对照组、蚓激酶小剂量组(2000IU/kg)和蚓激酶大剂量组(4000IU/kg)。经脚趾皮下注射1%角叉菜胶(5mg/kg)制作血栓模型,于造模后24h、48h分别测量黑尾长度,第49h分离肱动脉采血,测定凝血指标。结果:高剂量的蚓激酶能够降低角叉菜胶诱导的大鼠尾部血栓形成率,减小角叉菜胶诱导的大鼠尾部血栓平均长度;与模型对照组相比,高、低剂量组均能使凝血酶原时间(PT)、凝血酶时间(TT)延长,纤维蛋白原含量(Fib)下降,其中高剂量组PT显著延长(P<0.05),维蛋白原含量明显降低(P<0.05),差异具有统计学意义;高剂量的蚓激酶还能缓解注射角叉菜胶引起的体重减少。结论:蚓激酶具有预防和缓解血栓形成的作用,能够提高机体的纤溶功能。Objective：To evaluate the thrombolytic effect of Lumbrokinase （LK） from Eisenia foetida on thrombopoiesis of rats induced by carrageenan. Methods：Rats are randomly divided into normal control group, model control group, small dose lumbrokinase group （2000IU/kg） and high dose lumbrokinase group （4000IU/kg）. Thrombopoiesis at rats＆#39; tail is induced by subcutaneous injection of 1%carrageenan （5mg/kg）. Thrombus length are detected 24h and 48h respectively after injecting carrageenan, and humerus artery blood of rats are separated at 49th hour and blood coagulation index were measured. Results：The high dose LK can obivously decrease the black tail occurrence and the black tail length;Compared with model control group, both the low does LK and the high does LK can extend the prothrombin time （PT）, thrombin time （TT） and reduce the content of human fibrinogen （Fib）. In addition, PT length （P〈0.05） and Fib content reducing （P〈0.05） were significant in the high dose lumbrokinase group, and difference have statistical significance. The high dose lumbrokinase group could also relieve the lose of body weight induced by carrageenan. Conclusion：LK could prevent and relieve the thrombosis and improve the fibrinolytic function of body.

关 键 词：蚓激酶 溶栓 角叉菜胶 凝血指标 

分 类 号：R96[医药卫生—药理学]