Establishment of a highly efficient hematopoietic differentiation model from human embryonic stem cells for functional screening  

作　　者：PANG SuLei WU QingQing TIAN Sha SU Pei BAI Yang GAO Jie YANG YiQing LIU Xin ZHU ZhengMao XU YuanFu ZHOU JiaXi 

机构地区：[1]State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Diseases Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College [2]College of Life Sciences,Nankai University

出　　处：《Science China(Life Sciences)》2013年第12期1147-1149,共3页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China(2012CB966403);National Natural Science Foundation of China(31171431);Tianjin Natural Science Foundation(11JCYBJC27300)

摘　　要：Human embryonic stem cells （hESCs） have been successfully differentiated into hematopoietic progenitor cells with colony formation capacity and further into various kinds of blood cells including erythrocytes, megakaryocytes, neutrophils, nature killer cells and T lymphocytes . Nevertheless, the differentiation efficiency is extremely low. The differentiation usually involves complex steps such as embryonic body formation or co-culture with stromal cells, causing inconsistencies and difficulties in dissection of its molecular mechanisms [8,9]. Therefore, it is essential to establish a well-defined hematopoietic differentiation model that is independent of serum and stromal cells and much easier to manipulate genes for functional screening, thus the underlying molecular mechanism of hematopoietic differen- tiation of hESCs could be dissected more easily.Human embryonic stem cells(hESCs)have been successfully differentiated into hematopoietic progenitor cells with colony formation capacity and further into various kinds of blood cells including erythrocytes,megakaryocytes,neutrophils,nature killer cells and T lymphocytes[1 7].Nevertheless,the differentiation efficiency is extremely low.

关 键 词：人胚胎干细胞 造血祖细胞 分化 筛选 模型 人类胚胎干细胞 嗜中性粒细胞 自然杀伤细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]