Treg细胞在儿童过敏性哮喘SIT治疗过程中的变化  

Change of treg cells during house dust mites specific immunotherapy for children with allergic asthma

作　　者：朱俊民[1] 李付广[2] 

机构地区：[1]漯河市中心医院检验科,462000 [2]郑州大学基础医学院

出　　处：《中国实用医药》2013年第33期3-5,共3页China Practical Medicine

摘　　要：目的：本研究通过观察对室尘螨（HDM）过敏的儿童哮喘患者,过敏原特异性免疫治疗（allergen specific immunotherapy, SIT）后外周血CD4＋CD25＋CD127-Treg细胞（regulatory T cell, Treg）细胞）占CD4＋T cell的百分比的变化,以及它们在SIT治疗中的作用,初步探讨儿童过敏性哮喘SIT的机制,从而为哮喘的防治提供新思路。方法选取郑州大学第一附属医院儿科门诊48例儿童过敏性哮喘患者分两组,一组进行标准化SIT 1.5-2年后,采集两组患者的外周静脉血。用流式细胞仪检测每组标本中CD4＋CD25＋CD127-T细胞占CD4＋T细胞的百分比,结果采用统计学软件进行统计学分析。结果CD4＋CD25＋CD127-T细胞占CD4＋T细胞的百分比在HDM-SIT组显著增高。结论本实验进一步证实了哮喘患者SIT治疗有效；在HDM-SIT组CD4＋CD25＋CD127-T细胞占CD4＋T细胞的百分比升高,提示CD4＋CD25＋CD127-T细胞可能在儿童过敏性哮喘特异性免疫治疗中起重要作用。Objective The aim of this study is to observe house dust mite （HDM） allergic children with asthma allergen-specific immunotherapy （SIT） in peripheral blood CD4＋CD25＋CD127-Tcells accounted for the percentage of CD4＋T cells changes and their role in the SIT treatment, preliminary study of children with allergic asthma SIT mechanism, so as to provide new ideas for the prevention and treatment of asthma. Methods First Affiliated Hospital of Zhengzhou University pediatric clinic 48 cases of children with allergic asthma patients divided into two groups, one group for standardization SIT 1.5~2 years later, the two groups were collected peripheral blood. Each flow cytometry specimens CD4＋CD25＋CD127-T cells accounted for the percentage of CD4＋Tcells, the results were statistically analyzed using statistical software. Results CD4＋CD25＋CD127-Tcells accounted for the percentage of CD4＋T cells in HDM-SIT group was significantly higher. Conclusion The present study further confirmed SIT asthma treatment is effective;in HDM-SIT groupCD4＋CD25＋CD127-Tcells accounted for the percentage of CD4＋Tcells increasedCD4＋CD25＋CD127-Tcells, suggesting that CD4＋CD25＋CD127-T cells may be specifically allergic asthma in children immunotherapy plays an important role.

关 键 词：特异性免疫治疗 过敏性哮喘 CD4+CD25+CD127-细胞 CD4+T细胞 室尘螨 

分 类 号：R725.6[医药卫生—儿科]