实验性早期心肌缺血补体C5的免疫组织化学研究  

Immunohistochemical study of C5 complement in experimental early myocardial ischemia

作　　者：胡丙杰[1] 陈玉川[1] 祝家镇[1] 毕启明[1] 李杰 曾家乐 

机构地区：[1]中山医科大学法医学系,广州510089 [2]广东东莞市公安局法医室,东莞511700

出　　处：《中国法医学杂志》2000年第4期197-199,共3页Chinese Journal of Forensic Medicine

基　　金：卫生部科研基金!(96 -1-12 7);卫生部优秀青年科技人才专项科研基金!(970 12 );广东省自然科学基金!(980 0 78);广东省医药卫生

摘　　要：建立大鼠急性心肌缺血模型。应用免疫组织化学方法和图像分析技术 ,对急性心肌缺血早期不同缺血时间心肌细胞内补体C5的变化进行研究。结果发现 :C5在心肌缺血 15min即可出现阳性反应 ,并随缺血时间的延长 ,其反应面积逐步增大。心肌细胞内C5阳性是早期心肌缺血的敏感指标 。Experimental acute myocardial ischemia model of rat was established, and the changes of C5 complement in the ischemic myocardia were studied with immunohistochemistry and image analysis technique. The results showed that the positive reaction of C5 could be observed in ischemic myocardia at 15 min after ischemia, and the positive reaction area increased along with the prolongation of the ischemic period. It is concluded that the positive reaction of C5 in cardiomyocytes is a quite sensitive marker of early myocardial ischaemia.The immunohistochemical detection of C5 in cardiomyocytes will be a meaningful tool for the postmortem diagnosis of early myocardial ischaemia.

关 键 词：早期心肌缺血 补体 免疫组织化学 C5 实验研究 

分 类 号：R392.11[医药卫生—免疫学]