玉米ZmPEPC基因的克隆及其转化春小麦的研究  

Cloning of Zmpepc Gene from Maize and Transformation into Spring Wheat

作　　者：王重[1] 张跃强[1] 樊哲儒[1] 赵奇[1] 李剑峰[1] 张宏芝[1] 王子霞[1] 海热古丽.阿不力孜 

机构地区：[1]新疆农科院核技术生物技术研究所,新疆乌鲁木齐830091

出　　处：《核农学报》2013年第11期1617-1623,共7页Journal of Nuclear Agricultural Sciences

基　　金：新疆维吾尔自治区青年科学基因项目(2010211B29);新疆农业科学院青年基金项目(xjnkq-2013025)

摘　　要：磷酸烯醇式丙酮酸羧化酶(PEPC)是C4光合途径中的关键酶,为了研究该酶对春小麦光合特性的改良作用,本研究从玉米(Zea mays)中克隆了ZmPEPC基因,生物信息学分析表明该基因CDS全长2913 bp,编码的多肽链包含970个氨基酸,其中175-970位氨基酸组成了磷酸烯醇式丙酮酸羧化酶的功能结构域,在173-184、597-609位具有两个磷酸烯醇式丙酮酸羧化酶活性位点。将该基因通过基因枪法转化优质强筋小麦新春26号,对转基因春小麦的叶绿素含量及磷酸烯醇式丙酮酸羧化酶活性进行测定,结果显示转基因春小麦两项指标较非转基因春小麦均有提高,表明ZmPEPC基因对于春小麦光合特性的改良具有积极的作用,为C4光合途径关键酶在春小麦高产改良中的应用奠定了良好的基础。Phosphoenolpyruvate carboxylase （PEPC） is a key enzyme in the C4 photosynthetic pathway. In order to study the improved effect of the enzyme on photosynthetic characteristics in spring wheat, the ZmPEPC gene in maize （zea mays） was successfully cloned. The bioinformatics analysis showed that the coding sequence of the ZmPEPC gene had 2913 bp in length, encoding 970 amino acids. One functional domain of the phosphoenolpyruvate carboxylase at position 175-970, and two phosphate pyruvate carboxylase activity sites at position 173 -184 and 597 -609 were found. Through particle-mediated bombardment, the ZmPEPC gene was transferred into spring wheat Xinchun 26, which has good quality and strong gluten. Compared with non-transgenic control, the chlorophyll content and phosphoenolpyruvate carboxylase activity of transgenic spring wheat were increased. The result indicates the ZmPEPC gene plays an active role in improving the photosynthetic characteristics of spring wheat, and establishes a good foundation for using of the C4 photosynthetic pathway key enzyme in improving the yield of spring wheat.

关 键 词：玉米 春小麦 ZmPEPC 基因克隆 转化 

分 类 号：S512.12[农业科学—作物学]