基于小波变换的鸭蛋蛋清中卡那霉素残留含量的同步荧光光谱测定研究  

Study on Determination of Kanamycin Content in Duck Egg White Using Synchronous Fluoresence Spectrum and Wavelet Transform

作　　者：赵进辉[1] 袁海超[1] 刘木华[1] 徐将[1] 肖海斌[1] 洪茜[1] 

机构地区：[1]江西农业大学工学院/生物光电及应用重点实验室,江西南昌330045

出　　处：《核农学报》2013年第11期1686-1691,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31101295);江西省科技厅科技支撑项目(20121BBG70058);江西省教育厅科技计划项目(GJJ12244)

摘　　要：为实现鸭蛋蛋清中卡那霉素残留含量的快速测定与检测模型精度的提高,获取卡那霉素在巯基乙醇存在的条件下与邻苯二甲醛生成的衍生物的同步荧光光谱,应用小波分析结合e-SVR建立鸭蛋蛋清中卡那霉素残留含量的预测模型。在模型建立过程中,首先应用三维同步荧光法对波长290～380nm的同步荧光光谱进行分析,确定测定鸭蛋蛋清中卡那霉素残留含量的最佳波长差Δλ为110 nm。然后应用db5小波对荧光光谱进行5层小波分解,分析表明第4层小波细节信号能较好的解决荧光光谱重叠及卡那霉素微弱荧光信号提取问题,并确定以细节小波系数d4作为模型的输入特征向量。最后从核函数类型、参数(c,g,p)取值方面对e-SVR模型进行优化,获取RBF为最优核函数,进而对PLS、PCR、MLR和e-SVR 4种模型进行性能比较,以e-SVR模型的预测效果最好,其预测集的相关系数和均方根误差分别为0.9922和1.1865。结果表明,小波变换能有效提取出鸭蛋蛋清中卡那霉素的荧光特征信息,有利于提高e-SVR模型预测精度,能够满足鸭蛋蛋清中卡那霉素残留含量快速测定要求。To achieve the rapid prediction of kanamycin contents in duck egg white and improve accuracy of prediction model, synchronous fluorescence spectrum of derivate of kanamycin and o-phthalaldehyde （OPA） under the condition of mercaptoethanol was obtained, and the prediction model of kanamycin contents in duck egg white was developed combined with wavelet transform and e-SVR. In the process, synchronous fluorescence spectrum from 290 nm to 380 nm was analyzed using three-dimensional synchronous fluorescence method, and 110 nm was selected as the optimum wavelength difference for determination of kanamycin content in duck egg white. Secondly, fluorescence spectrum was analyzed using the db5 wavelet with five levels decomposition, the analysis showed that wavelet details signal of the fourth level could better solve problems that the synchronous fluorescence spectrum overlapping and the extraction of weak fluorescence signal of kanamycin, so the detail wavelet coefficient d4 was selected as the input characteristic vectors of detection model. Lastly, the e-SVR model was optimized from the two aspects of kernel functions and model parameters （c, g, p）, and RBF was selected as kernel function. Furthermore, the performances of 4 models of PLS PCR,MLR and e-SVR were compared. The best prediction results were obtained using the e-SVR model, and the correlation coefficient and the root mean squared error of prediction were 0. 9922 and 1. 1865, respectively. The experimental results showed that wavelet transform could extract effectively fluorescence characteristic information of kanamycin in duck egg white and improve the prediction accuracy of e-SVR model. The proposed method in the paper could satisfy the request of determination of kanamycin content in duck egg white.

关 键 词：同步荧光光谱 小波变换 支持向量回归 卡那霉素 

分 类 号：S859.84[农业科学—临床兽医学]