玉米苗期受低温胁迫蛋白表达差异研究  

Differentially Expressed Proteome under Low Temperature Stress in Maize Seedling

作　　者：杨猛[1] 庄文锋[2] 魏湜[1] 顾万荣[1] 杨晔[1] 王萌[1] 李晶[1] 

机构地区：[1]东北农业大学农学院,哈尔滨黑龙江150030 [2]哈尔滨市农业科学院,哈尔滨黑龙江150070

出　　处：《核农学报》2013年第11期1742-1748,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家科技支撑计划(2011BAD32B03-02);寒地作物品种改良与生理生态重点开放实验室项目(hdzw-008);黑龙江省博士后基金项目(LBH-Z10253)

摘　　要：为寻找玉米苗期抗低温表达调控相关蛋白。本试验应用双向电泳技术对吉单198、兴垦3和金玉5 3个品种不同温度处理全蛋白进行分析,结果表明:232个蛋白点上调,156个蛋白点下调,28个新增蛋白点,3个消失蛋白点,对其中42个丰度大于2、差异强度大的蛋白质点采用MALDA-TOF-MS进行肽指纹图谱分析,经数据库搜索匹配,鉴定出8个未知蛋白、7个假定蛋白和27个功能蛋白,这些蛋白参与防御反应、能量代谢、光合作用、蛋白降解、核糖体形成、信号传导、细胞运动、氮代谢等多个生理过程,实现玉米幼苗在低温条件下的代谢平衡。The purpose of this experiment was to discover regulation mechanism for key protein expression. The varieties of ＇ Jidan 198 ＇ , ＇ Xingken 3 ＇ and ＇ Jinyu 5＇ were used to analyze the leaf total protein of maize seedlings based on 2- DE. Results showed that 232 up-regulated protein spots, 156 down-regulated protein spots, 28 new adding protein spots and 3 protein spots disappeared under low-temperature stress. MALDI-TOF-MS analysis showed that 8 unknown proteins, 7 assuming proteins and 27 functional proteins were detected. These proteins were relevant to some physiology procedures, such as defense reaction, energy metabolism, photosynthesis, protein degradation, ribosome formation, signal conduction, cell movement and nitrogen metabolism, which were beneficial for the metabolism balance of maize seedling under low temperature stress.

关 键 词：玉米 低温胁迫 蛋白表达 双向电泳 

分 类 号：S513[农业科学—作物学]