我国内脏利什曼病山丘疫区与平原疫区利什曼原虫SSUrDNA多变区序列分析  

SEQUENCE ANALYSIS OF SSU rDNA VARIABLE REGIONS OF LEISHMANIAISOLATES FROM HILLY FOCI AND PLAIN FOCI OF CHINA

作　　者：卜玲毅[1] 胡孝素[1] 敬保迁[2] 易桃林[1] 

机构地区：[1]华西医科大学寄生虫学研究室,成都610044 [2]川北医学院微生物学教研室,南充637000

出　　处：《中国寄生虫学与寄生虫病杂志》2000年第6期321-324,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金资助项目!(No .39970 6 6 7)

摘　　要：[目的 ]分析我国内脏利什曼病 (VL)山丘疫区与平原疫区利什曼原虫 (L .d )分离株小亚基核糖体DNA (SSUrDNA)多变区的序列差异。 [方法 ]nDNA进行PCR扩增 ,将扩增出的SSUrDNA基因的特异片段克隆于 pGEMR TEasyVector上 ,采用通用引物M 13进行PCR扩增 ,全自动测序仪测序。 [结果 ]序列分析显示 ,扩增的 5株利什曼原虫SSUrDNA序列大小均为 392bp ;序列差异均发生在两个独特序列区 (UQ Ⅰ和UQ Ⅱ ) ;山丘疫区L .d甘肃分离株和四川分离株均在UQ Ⅱ区上有 2个相同的碱基突变 ,L .d甘肃分离株UQ Ⅰ区上有 1个碱基突变 ;无移码突变。 [结论 ]山丘疫区分离株与平原疫区分离株之间的碱基序列有差异 ,平原疫区L .d山东分离株与婴儿利什曼原虫 (L .infantum)Objective] To analyze the sequence difference of the SSU rDNA variable regions of Leishmania isolates from hilly foci and plain foci of China. [Methods] Specific SSU rDNA fragments from nuclear DNA of five Leishmania species and isolates were amplified by PCR. The amplified DNA fragments were cloned into pGEM R\|T Easy vector. The specific fragments were sequenced by the automated DNA sequencer. [Results] Sequence analysis showed that the amplified DNA fragments of five Leishmania species and isolates were all 392 bp in length, point mutations were located in the two unique sequence (UQ\|Ⅰ and UQ\|Ⅱ); L.d.SC10 and L.d.GS7 had two same point mutations in UQ\|Ⅱ, only L.d.GS7 had one in UQ\|Ⅰ; no insertion/deletion. [Conclusion] Sequence difference of the SSU rDNA variable region existed between Leishmania isolates from hilly foci and plain foci; The sequences of the SSU rDNA variable regions of L.d. SD2 isolate and L.infantum were identical.

关 键 词：内脏利会曼病 利会曼原虫 SSUrDNA 序列分析 

分 类 号：R531.6[医药卫生—内科学] R382.22[医药卫生—临床医学]