我国鸡传染性支气管炎病毒地方分离株的形态观察和S1基因的RT-PCR扩增  

EM Observation and RT-PCR of the S1 Gene of Five Chinese Isolate of Avian Infectious Bronchitis Virus

作　　者：游洪[1] 王林川[1] 

机构地区：[1]华南农业大学动物医学系,广州510642

出　　处：《江西农业大学学报》2000年第4期594-598,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金资助项目! (398932 90 - 2B)

摘　　要：参照已发表的IBVBeaudette株全基因组序列 ,自行设计合成了 3条引物 (youl+、you2 -、youM + ) ,引物you1+和you2 -在S1基因两侧 ,跨幅约 16 10bp ,引物you2 -和youM +在S1基因的 3’端 ,跨幅约 5 30bp。用这两对引物对 5个IBV地方分离株 (HN2 ,HN4,JX1,SC2和SC1)进行RT -PCR ,均成功地扩增出相应大小的目的片段。According to the sequence of the genome of the Beaudette strain of IBV, three primers (you1+、you2- and youM+) were designed and synthesized. The primers you1+ and you2- locate respectively in the 5'-most and the 3'-most of the S1 gene and approximately cover 1610bp; the primer youM+ lies in the middle of the S1 gene. RT-PCR was used to amplify the S1 gene fragment of the five Chinese isolates of IBV (HN2, HN4, JX1, SC2 and SC1) using the three primers. Two special PCR fragments (1610bp and 530bp) were observed respectively. The virus were observed with transmission electron microscope (EM), and a lot of viral particles were seen.

关 键 词：鸡 传染性支气管炎病毒 病毒形态 S1基因 RT-PCR 

分 类 号：S858.315.3[农业科学—临床兽医学]