山东省小麦赤霉病菌对多菌灵的敏感性测定  

Sensitiveness of Fusarium graminearum to Carbendazim in Shandong Province

作　　者：孙玲[1] 张洪滨[1] 刘秉江 徐加利[3] 梁元存[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]山东省新泰市农业局,山东新泰271200 [3]山东省泰安市农业局,山东泰安271000

出　　处：《农药》2013年第12期914-916,共3页Agrochemicals

基　　金：国家自然科学基金资助项目(31171806);山东省现代产业技术体系建设经费资助

摘　　要：[目的]测定山东省小麦赤霉病菌(Fusarium graminearum)对多菌灵的敏感性水平,为生产中合理用药提供参考。[方法]以2.0 mg/L多菌灵为鉴别剂量,在PDA平板上测定90个菌株的敏感性。用菌落直径法测定菌株的EC50值并计算抗性倍数;用孢子萌发法测定10 mg/L多菌灵对芽管伸长的抑制情况。[结果]90个小麦赤霉菌株的EC50平均值为(0.5269±0.0093)mg/L,没有抗性菌株存在;10 mg/L多菌灵对芽管伸长有明显抑制作用并导致芽管畸形。[结论]山东省小麦赤霉病菌均为多菌灵的敏感菌株,多菌灵可用于防治小麦赤霉病。[Aims] The study aims to determine sensitivity to carbendazim （MBC） in Fusarium graminearum, and to provide reference for agricultural production in Shandong province. [Methods] A total of 90 isolates of E graminearura were collected from 15 districts in Shandong province to determine the sensitivity to MBC with 2.0 mg/L as the distinctive concentration. The values for 50% effective concentration （EC,0） were identified by measuring mycelial growth in fungicide- amended PDA media and the resistance ratio was counted. The inhibitory effect of 10 mg/L MBC on E graminearum isolates was also investigated by spore germination assay. [Results] These results showed that the mean EC^0 value of 90 isolates was （0.526 9~0.009 3） mg/L, there was no MBC-resistant isolate in Shandong province. Spore germination assay showed that 10 mg/L of MBC significantly affect the elongation of germ tube and resulted in abnormal growth. [Conclusions] All isolates ofE gram/nearum were sensitive to MBC, and MBC could be used in Shandong province.

关 键 词：小麦赤霉病菌 多菌灵 抗药性 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]