离子选择性电极技术快速检测透明质酸钠  

Potentiometric measurement of Sodium Hyaluronate by using a polymeric membrane ion-selective electrode

作　　者：陈燕[1] 李雪梅[1] 李秋顺[1] 史建国[1] 

机构地区：[1]山东省科学院生物研究所,山东省生物传感器重点实验室,济南250014

出　　处：《分析试验室》2013年第12期42-45,共4页Chinese Journal of Analysis Laboratory

基　　金：国家"863"计划项目(2012AA021201);山东省自然科学基金项目(ZR2012CM029)资助

摘　　要：以聚阴离子透明质酸钠为研究对象，以二壬基萘磺酸为阳离子交换剂，发展了基于鱼精蛋白可控释放的聚阳离子选择性电极，实现了透明质酸钠的快速、高灵敏检测。详细研究了亲脂性有机盐四（4一氯苯基）硼酸四（十二烷基）铵对电极响应性能的影响。在最佳条件下，电极对透明质酸钠的检出限为0．05μg／mL，线性范围为0．1～5μg/mL。A polycation-selective polymeric membrane electrode using dinonylnaphthalene sulfonate as an ion exchanger has been developed as a protamine controlled-release system for potentiometric detection of sodium hyaluronate （SH）. The incorporation of tetradodecylammonium tetrakis （4-ehlorophenyl）borate as a lipophilic salt in the membrane dramatically improves selectivity of the sensor selectivity towards protamine over sodium ions via influencing the activity coefficient of protamine in the membrane and a stable potential baseline is obtained in the presence of an electrolyte background. Under optimal conditions, the proposed polymeric membrane electrode exhibits a linear range of 0. 1 - 5 relationship between the initial slope of the potential change and the SH concentration in the μg/mL with an improved detection limit of 0. 05 μg/mL.

关 键 词：离子选择性电极 离子通量 透明质酸钠 聚阳离子 

分 类 号：O657.1[理学—分析化学]