吖啶橙分光光度法测定双酚A  

Study on determination of bisphenol a by spectrophotometry with acridine orange

作　　者：赵冰冰[1] 高翠丽[1] 王亦军[1] 朱安宁[1] 

机构地区：[1]青岛大学化学化工与环境学院,青岛266071

出　　处：《分析试验室》2013年第12期51-54,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(50678085)资助

摘　　要：在酸性介质中，双酚A对羟基自由基氧化吖啶橙的反应具有抑制作用，据此建立了测定双酚A含量的分光光度分析方法。确定了最佳实验条件，在此条件下得到吖啶橙溶液吸光度的变化值与双酚A浓度的线性关系，其线性范围为10—200ng／mL，工作曲线为△A=0．643logρ-0．544，线性相关系数为0．9991，检出限为1．05ng／mL。应用本法测定4种塑料制品中双酚A的含量，回收率在95．0％～104．3％之间，RSD在1．7％～3．1％之间。A new spectrophotometric method for the determination of bisphenol A was established based on inhibitory effect of bisphenol A on the oxidation of acridine orange with hydroxyl radical from Fenton reaction in acid medium. The optimum experimental conditions were investigated, and the results were as follows： there was a linear relationship between the absorption change of acridine orange and the concentration of bisphenol A, the linear range of determination of bisphenol A was 10 - 200 ng/mL with the linear regression equation as△A = 0. 6431ogρ- 0. 544, and the detection limit was 1.05 ng/mL. The method was used for the determination of bisphenol A in 4 samples, the relative standard deviations were 1.7% - 3.1% and the recoveries were 95.0% - 104.3% with satisfactory results.

关 键 词：吖啶橙 双酚A FENTON试剂 分光光度法 

分 类 号：O657.3[理学—分析化学]