健脾化湿颗粒对D-IBS模型大鼠内脏敏感性和脑中CRF及其受体的影响  

Effect of Jianpi Huashi Granule on Visceral Sensitivity and CRF/CRF Receptors in Brain of D-IBS Model Rat

作　　者：杜海燕 王迎寒 张晓峰 靳继伟 郭洪杰[2] 

机构地区：[1]河北省中药研究与开发重点实验室,承德067000 [2]承德医学院肛肠医院,承德067000

出　　处：《实验动物科学》2013年第5期15-19,共5页Laboratory Animal Science

基　　金：河北省科学技术研究与发展计划项目(No.10276105D-45)

摘　　要：目的观察健脾化湿颗粒对腹泻型肠易激综合征(DIARRHEA-PREDOMINANT of Irritable Bowel Syndrome,D-IBS)模型大鼠内脏敏感性和脑中促肾上腺皮质激素释放因子(Corticotropin-Releasing Factor,CRF)及促肾上腺皮质激素释放因子受体1(Corticotropin-Releasing Factor Receptor 1,CRF-R1)mRNA表达的影响。方法 72只SD大鼠随机分为正常组、模型组、得舒特组和健脾化湿颗粒低、中、高剂量组;用番泻叶灌胃结合束缚应激法复制D-IBS大鼠模型;采用直肠扩张腹壁撤退反射(Abdominal With drawal Reflex,AWR)评分评价大鼠的内脏敏感性;采用PCR法检测大鼠脑中CRF、CRF-R1 mRNA的表达。结果与正常组相比,模型组AWR评分为3的注水量明显降低(P<0.01),脑中CRF、CRF-R1 mRNA的表达明显升高(P<0.01);与模型组比较,健脾化湿颗粒中、高剂量组、得舒特组AWR评分为3的注水量明显升高(P<0.01),脑中CRF、CRF-R1mRNA的表达明显降低(P<0.01)。结论健脾化湿颗粒中、高剂量组可降低大鼠的内脏敏感性,其作用机制可能与降低中枢CRF及CRF-R1 mRNA的表达有关。Objective To explore the effects of Jianpi Huashi Granule on visceral sensitivity and CRF/CRF-R1expression in brain of D-IBS model rat.Method Seventy-two male Sprague-Dawley rats were equally and randomly divided into six groups：normal group,model group,dicetel group,low-,medium-,and high-dose Jianpi Huashi Granule groups; D-IBS was induced by restraint stress combined with administration of sennae decoction; The visceral sensitivity of rats was evaluated by colorectal distention,and recorded the water injection rate when the score of abdominal withdrawal reflex （AWR） was 3.The expression of CRF and CRF-R1mRNA in the brain was detected by PCR.Result Compared with normal group,the water injection rate of the model group showed a significant decrease when the score of AWR was 3 （P ＜ 0.01）,and the expression of CRF and CRF-R1 mRNA in the brain significantly increased （P ＜ 0.01） ; compared with model group,the water injection rate of the dicetel group,medium-,and high-dose Jianpi Huashi Granule groups showed a significant increase when the score of AWR was 3 （P ＜ 0.01）,and the expression of CRF and CRF-R1 mRNA in the brain significantly decreased （P ＜ 0.01）.Conclusion medium-,and high-dose Jianpi Huashi Granule groups reduces visceral sensitivity in the rats with D-IBS possibly by modulating the expression of CRF and CRF-R1mRNA in the brain.

关 键 词：健脾化湿颗粒 肠易激综合征 内脏敏感性 CRF CRF-R1 

分 类 号：Q95-33[生物学—动物学]