氧化鱼油对鲤肝胰脏抗氧化机能及其组织结构的影响  

Effects of oxidized fish oil on the anti oxidative function and histological structure of carp hepatopancreas

作　　者：任泽林[1] 曾虹[1] 霍启光[1] 周文豪[1] 范志影[1] 

机构地区：[1]中国农业科学院饲料研究所,北京100081

出　　处：《大连水产学院学报》2000年第4期235-243,共9页Journal of Dalian Fisheries University

摘　　要：在半纯化饲料中添加质量分数为 3%的新鲜鱼油 (过氧化物值POV为 0 64mmol/kg) 和POV分别为 2 9 64、 59 40和 94 69mmol/kg (以O2 计 )的氧化鱼油 ,投喂1 0 0 g左右 2龄鲤 (Cyprinuscarpio)鱼种 1 5周 ,结果表明 :鲤摄食氧化鱼油后 ,肝胰脏维生素E含量下降 (P <0 0 5) ,抗氧化酶超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH -Px)活性减弱 (P >0 0 5) ,微粒体氧化稳定性降低 (P <0 0 5) ,鱼油氧化程度升高 ,上述变化加强 ;肝胰脏脂肪含量增加 (P >0 0 5) ;肾脏和脾脏增生 (P >0 0 5) ;肝胰脏肝细胞纤维化、线粒体嵴降解和融合。上述系列变化表明氧化鱼油可以破坏鲤肝胰脏抗氧化机能及其正常的组织结构。Juvenile carp (\%Cyprinus carpio\%) weighing 100 g were fed a semi purified diet supplemented with 3% of fresh fish oil (Peroxide value, POV, 0.64 mmol/kg) or oxidized fish oil at POV levels of 29.64, 59.40, or 94.69 mmol/kg for 15 weeks. Oxidized fish oil decreased hepatopancreas vitamin E contents (\%P\%<0.05) and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) (\%P\%>0.05) and microsomal oxidative stabilities (\%P\%<0.05). These trends were even worse when oxidized degrees of fish oil increased. With the oxidized fish oil used in the diet, fat content in hepatopancreas was elevated (\%P\%>0.05) and kidney somatic indices (KSI) and spleen somatic indices (SSI) were enhanced (\%P\%>0.05). In hepatopancreas of carp fed diet with oxidized fish oil, pronounced histological abnormalities were noted including liver cell fibrillation and degenerated and melted cristae of mitochondria. These changes reveal that oxidized fish oil destroyed anti oxidative function and normal histological structure of hepatopancreas.

关 键 词：鲤 氧化鱼渔 肝胰脏 抗氧化机能 组织结构 饲料 

分 类 号：Q959.468[生物学—动物学] S965.116[农业科学—水产养殖]