联合水蛭注射液后处理对大鼠脑缺血损伤磷酸化蛋白激酶B表达的影响  

Effect of combined leech injection and ischemic postconditioning on expression of p-Akt in rats following I/R injury

作　　者：胡跃强[1] 唐农[2] 吴林[2] 孙淑荣[1] 苏锦勋[2] 覃琴[1] 

机构地区：[1]广西中医药大学第一附属医院神经内科,南宁530023 [2]广西中医药大学

出　　处：《中华老年心脑血管病杂志》2013年第12期1321-1323,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：广西自然科学基金(2012GXNSFAA053075);广西卫生厅重点课题(2012046)

摘　　要：目的观察脑缺血后处理中,联合水蛭注射液进行后处理,对脑缺血损伤大鼠磷酸化蛋白激酶B mRNA及其蛋白表达的影响。方法 SD大鼠40只,随机分为假手术组(SO组)、缺血再灌注组(I/R组)、缺血后处理组(IPOC组)、IPOC+水蛭注射液后处理组(P-L组),每组10只。采用线栓法制备大鼠大脑中动脉闭塞模型及IPOC模型,应用实时荧光定量PCR和Western blot法检测磷酸化蛋白激酶mRNA及其蛋白表达。结果 SO组有少量磷酸化蛋白激酶mRNA及蛋白表达;与SO组比较,I/R组表达明显升高;与I/R组比较,IPOC组表达明显升高(P<0.01);与IPOC组比较,P-L组表达明显升高(P<0.05)。结论 IPOC可能通过诱导磷酸化蛋白激酶的表达发挥其神经保护作用,水蛭注射液可进一步促进其表达而发挥神经保护作用。Objective To study the effect of combined leech injection and ischemic postconditioning on expression of p-Akt mRNA and protein in rats following I/R injury. Methods Forty male SD rats were randomly divided into sham operation group, I/R group, ischemie posteonditioning group and leech injection postconditioning group （10 in each group）. A middle cerebral artery oc- clusion model and an isehemie posteonditioning model were established by Longa occlusion. Ex- pression of p-Akt mRNA and protein was detected by RT-PCR and Western blot, respectively. Results Only a mall amount of p-Akt mRNA and protein was expressed in sham operation group. The expression level of p-Akt mRNA and protein was significantly higher in I/R group than in sham operation group,in ischemie postconditioning group than in I/R group, and in leech injection postconditioning group than in ischemie postconditioning group （P〈0.05）. Conclusion Ischemic posteonditioning can protect nerves by inducing p-Akt expression and leech injection postconditioning plays an important role in protection of nerves by further promoting its expression.

关 键 词：药用水蛭 脑缺血 蛋白激酶类 再灌注损伤 RNA 信使 

分 类 号：R743[医药卫生—神经病学与精神病学]