A new type of immobilized metal ion affinity chromatography(IMAC) adsorbent for highly specific enrichment of phosphopeptides for phosphoproteomics analysis  

出　　处：《Science Foundation in China》2013年第1期58-58,共1页中国科学基金（英文版）

基　　金：funded by NSFC

摘　　要：Phosphorylation is a key reversible modification of proteins that acts as a switch to regulate almost all biological processes.Phosphoproteomics analysis aims to identify and characterize all the protein phosphorylations presented in cells or tissues.Conventional adsorbents for phosphopeptide enrichment lack enough specificity,which seriously compromises the performance of phosphoproteome analysis.The research lab headed by Prof.Zou Hanfa in CAS Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences(CAS)developed a new type of immobilized metal ion affinity chromatography(IMAC)adsorbent for highly specific enrichment of phosphopeptides in phosphoproteomics analysis.The schematics for this new IMAC are shown in the Figure below.The phos-Phosphorylation is a key reversible modification of proteins that acts as a switch to regulate almost all biological processes. Phosphoproteomics analysis aims to identify and characterize all the protein phospho- rylations presented in cells or tissues. Conventional adsorbents for phosphopeptide enrichment lack enough specificity, which seriously compromises the performance of phosphoproteome analysis.

关 键 词：磷酸化蛋白质组 吸附剂 IMAC 亲和层析 金属离子 富集 异性 学分 

分 类 号：Q51[生物学—生物化学] O657.7[理学—分析化学]