温度对相同引种地三个秋茄居群遗传多样性的影响  

The Effects of Temperature on the Genetic Diversity of Three Kandelia Candel Populations in Sympatric Introducing Site

作　　者：杨娟[1] 马小伟 章宏琼[2] 周云[2] 宋佳楠[2] 朱忆思梦[2] 张洪勤[2] 施孟如[3] 

机构地区：[1]杭州师范大学发育与再生研究所,浙江杭州310036 [2]温州医科大学仁济学院,浙江温州325035 [3]温州医科大学生物学实验教学中心,浙江温州325035

出　　处：《杭州师范大学学报（自然科学版）》2013年第6期544-549,共6页Journal of Hangzhou Normal University(Natural Science Edition)

摘　　要：秋茄是世界分布最北的红树植物,研究温度对其遗传多样性的影响有助于阐明其抗冻机制.文章同时应用RAPD和ISSR分子标记技术,对3个不同纬度地区秋茄居群的遗传多样性和遗传分化进行分析,根据RAPD和ISSR数据计算遗传距离并进行聚类分析.2种方法均显示3个居群分为2大支:西门岛和象山港两居群之间遗传距离最小,两者之间的遗传一致度最大,首先聚为一类;漳江口和象山港居群之间的遗传距离最大,两者之间的遗传一致度最小,因此单独为一支.由于温度的原因对引种的秋茄进行了筛选,导致遗传多样性减少.Kandelia cande! is the northernmost distribution of mangrove in the world. The study about the effects of temperature on the genetic diversity of three Kandelia eande! populations in sympatric introducing site is useful to clarify the antifreeze mechanism. RAPD and ISSR molecular markers were used to detect the genetic diversity and genetic differentiation of three Kandelia candel populations in three different latitudes, and the genetic distances were calculated and clustering analyzed with the data of RAPD and ISSR. Both of the two methods showed that these three populations were separated to two groups. Ximen Island and Xiangshangang populations were clustered into one group, because the smallest genetic distance and the maximum genetic identity were found between them. Zhangjiangkou population was clustered into a group, because the largest genetic distance and the minimum genetic iaentity were found between Zhangjiangkou and Xiangshangang populations. The reason why the genetic diversity drops off is that the fall of temperature.

关 键 词：秋茄 RAPD ISSR 遗传多样性 

分 类 号：Q948[生物学—植物学]