欧美杨107杨β-1,3-葡聚糖酶(BG2)基因遗传转化及对溃疡病的抗性分析  

Transformation of BG 2 Gene into Populus × euramericana cv. ‘Neva'and Resistance of the Transgenic Poplar to Canker Disease

作　　者：牛庆霖[1] 王迎[2] 罗磊[2] 黄艳艳[2] 刘静[2] 冯殿齐[1,2] 曹帮华[1] 

机构地区：[1]山东农业大学农业生态与环境重点实验室,泰安271018 [2]泰安市泰山林业科学研究院,泰安271000

出　　处：《林业科学》2013年第11期60-66,共7页Scientia Silvae Sinicae

基　　金：山东省科技发展计划重点项目(2009GG10009002)

摘　　要：以欧美杨107杨为受体材料,利用根癌农杆菌介导法转化β-1,3-葡聚糖酶基因(BG2),经卡那霉素筛选,PCR和Southern检测显示有4个转基因阳性株系,初步证明外源目的基因已整合到107杨基因组中。离体抑菌试验表明转基因107杨(T-107杨)细胞粗提液的抑菌能力明显强于未转基因对照107杨(C-107杨)。在接种杨树溃疡病菌的培养基上培养,T-107杨长势明显强于C-107杨;T-107杨可明显抑制丙二醛(MDA)在植物体内的快速积累,其苯丙氨酸解氨酶(PAL)含量也明显高于C-107杨,PAL含量T-107杨和C-107杨都有先升后降的趋势;15天后C-107杨完全死亡,T-107杨仍能生存。试验结果表明BG2基因的表达提高了107杨对杨树溃疡病的抗性。The BG2 gene was transformed into Populus x euramericana cv. ＇ Neva＇ via Agrobacterium tumefaciens mediated transformation method. Four transgenic lines were obtained through selection of kanamycin resistance, PCR and Southern analyses. These analyses suggested that the BG2 gene was integrated into the plant genome. Then, the transgenic plants were tested for resistance to the fungal pathogen Cytospora chrysosperma. Crude extracts of the transgenic T-107 plants exhibited in vitro inhibitory activity against the fungal pathogen. When inoculated with Populus euramericana canker disease, the T-107 plants grew better than the C-107 plants. The MDA levels of T-107 were lower than that in C-107. The PAL contents of both transgenic and control plants were firstly increased obviously then decreased; However, the PAL content of T-107 plants was always higher than that in C-107 plants. After 15 days, all the C-107 plants dead, but the T-107 was still alive. These results indicated that the transformed BG2 gene could improve the resistance of T-107 plants to canker disease.

关 键 词：欧美杨107杨 根癌农杆菌 BG2基因 基因转化 溃疡病 

分 类 号：S718.46[农业科学—林学]