快速起搏大鼠心房肌细胞内皮素转化酶-2表达变化  

Expressions of endothelin-converting enzyme-2 in primary rats with cultured atrial myocytes after rapid electrical-field stimulation

作　　者：刘艳霞[1] 张坡[2] 朱鲜阳[2] 韩秀敏[2] 王琦光[2] 张端珍[2] 

机构地区：[1]沈阳军区总医院干二科,沈阳110016 [2]沈阳军区总医院全军心血管病研究所先天性心脏病内科,沈阳110016

出　　处：《岭南心血管病杂志》2013年第6期733-736,共4页South China Journal of Cardiovascular Diseases

基　　金：辽宁省博士启动基金资助项目(项目编号:20081043)

摘　　要：目的利用原代培养的心房肌细胞建立快速起搏模型,研究内皮素转化酶-2在快速起搏早期的表达变化。方法原代培养大鼠心房肌细胞,并建立快速起搏细胞模型,利用反转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)和Western blot方法检测内皮素转化酶-2在快速起搏0、3、6、12、24 h后mRNA和蛋白表达的变化。结果内皮素转化酶-2的mRNA和蛋白0、3、6、12、24 h表达的相对值分别为:0.46±0.10、0.58±0.11、0.91±0.12、1.10±0.16、1.30±0.10及0.52±0.01、0.58±0.02、0.79±0.03、1.10±0.05、1.20±0.05。快速起搏3 h后内皮素转化酶-2的mRNA和蛋白表达较起搏前持续增加,差异均有统计学意义(P<0.05)。结论快速起搏早期,原代培养心房肌细胞内皮素转化酶-2的mRNA和蛋白表达量随起搏时间增加,提示内皮素转化酶-2和内皮素-1参与了心房颤动时心房病理重构。Objectives To study the expressions of endothelin-converting enzyme-2 （ECE-2） at early stages of atrial fibrillation in a rapid paced primary cultured atrial myocyte model. Methods Primary rat atrial myocytes were cultured and a rapid paced cell model was established. The atrial cells were divided into five groups with pacing durations of 0,3,6,12, 24 h. Reverse transcription polymerase chain reaction （RT-PCR） and Western blot（WB） were applied to detect the messenger ribonucleic acid（mRNA） and proteins expression of ECE-2. Results Relative magnitude of mRNA expression and protein of ECE-2 with pacing durations of 0, 3, 6, 12, 24 h were 0.46±0.10, 0.58±0.11, 0.91±0.12, 1.10±0.16, 1,30±0.10and 0.52±0.01, 0.58±0.02, 0.79±0.03, 1.10±0.05, 1.20±0.05, respectively, mRNA expression of ECE-2 increased after 3 h of rapid pacing and continued to elevate in a time dependent manner when compared with 0 h, the differences were significant （P〈0.05）. Similarly, changes of ECE-2 protein were paralleled with mRNA expression. Conclusions Expressions of ECE-2 increased in early phase of rapid paced atrial myoeytes. It implieats the ECE-2 and endothlin-1 plays a role in pathological remodeling of atrial fibrillation.

关 键 词：心房颤动 心肌 重构 内皮素转化酶-2 

分 类 号：R541.7[医药卫生—心血管疾病]