棘孢木霉菌对山新杨移栽苗叶片光合特性的影响  

Effects of Trichoderma asperellumon Leaf Photosynthesis of Tissue-cultured Populus davidiana×P.bolleana Loucne Seedlings Transplanted into Pots

作　　者：王慧[1] 刘志华[2] 吕曼曼[1] 唐磊[3] 朱国栋[1] 张荣沭[1] 

机构地区：[1]东北林业大学园林学院,黑龙江哈尔滨150040 [2]东北林业大学林学院,黑龙江哈尔滨150040 [3]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《北方园艺》2013年第23期78-82,共5页Northern Horticulture

基　　金：黑龙江省自然科学基金资助项目(C201216);国家自然科学基金资助项目(31170601);黑龙江省博士后科研启动基金资助项目(LBH-Q11176)

摘　　要：以山新杨组培移栽苗为试材,采用盆栽试验,研究了3个水平的棘孢木霉ACCC30536分生孢子悬液T1(5×102 cfu/cm3土)、T2(5×103 cfu/cm3土)和T3(5×104 cfu/cm3土)根施对杨树苗光合作用日变化和光响应曲线参数的影响。结果表明:T2水平木霉分生孢子对杨树苗的诱导效果最佳,净光合速率(Pn)的日变化呈单峰型并与CK一致,在12:30左右达到峰值后逐渐降低,且净光合速率高于CK和其它处理;此处理杨树苗的蒸腾速率(Tr)、气孔导度(Cond)和胞间CO2浓度(Ci)的日进程均与CK一致,且均高于CK;而且T2处理条件下杨树移栽苗的光响应曲线参数最大净光合速率(Pnmax)、表观量子效率(AQY)、和光饱和点(LSP)均高于CK和其它处理。说明根施棘孢木霉分生孢子悬液对杨树苗的光合作用具有积极的影响,最佳诱导量为5×103 cfu/cm3土。Taking tissue-cultured seedlings of Populus davidiana X P. bolleana Loucne as experimental material, through pot experiment,the effects of root application of Trichoderr, va asperellum ACCC30536 conidia of T1 （5×10^2 cfu/cm3cfu/cm3） ,T2 （5 ×10^3 cfu/cm3 soil） and T3 （5×10^4 cfu/cm3 soil） on photosynthetic diurnal variation and light response curve parameters of P. davidiana X P. bolleana Loucne seedlings were studied. The results showed that T2 inducing had the best effects. In T2 induced seedlings,net photosynthetic rates （Pn） were higher than CK and T1, T3 induced seedlings. The daily net photosynthetic rates curve showed one peak,which coincided with that of the CK and reached peak at about 12：30,after which the curve gradually went downwards. In T2 induced seedlings,daily curves of transpiration rate （Tr）, stomatal conductance （Cond）,intercellular COe concentration （Ci） were all in accordance with control while the values were higher than CK. In T2 induced seedlings, the light response curve parameters namely the maximum net photosynthetic rate （Pnmax）,apparent quantum efficiency （AQY）,light saturation points （LSP） were all higher than those of CK and T1, T3 induced seedlings. All these results led to a conclusion that T. asperellum spore suspension had a positive influence on the photosynthesis of P. davidiana X P. bolleana Loucne seedlings and the best inducing concentration was 5 ×10^3cfu/cma soil.

关 键 词：棘孢木霉 山新杨 光合作用 光响应特性 

分 类 号：S792.11[农业科学—林木遗传育种]