部分门静脉动脉化对梗阻性黄疸大鼠肝门部胆管的影响  

Effect of partial vein arterialization on hepatohilar bile duct in rats with obstructive jaundice

作　　者：辛宪磊[1] 陈永亮[1] 陈明易[1] 李崇辉[1] 李鸾[1] 冯健[1] 郭少华[1] 张安红[1] 

机构地区：[1]解放军总医院肝胆外科研究所,北京100853

出　　处：《解放军医学院学报》2013年第11期1178-1181,共4页Academic Journal of Chinese PLA Medical School

基　　金：北京自然科学基金项目(7112125)~~

摘　　要：目的探讨部分门静脉动脉化重建胆管血流后对梗阻性黄疸大鼠肝门部胆管、肝功能及胆管上皮的影响。方法 40只大鼠随机分为对照组(A)、梗阻性黄疸5 d行胆管再通组(B)、梗阻性黄疸5 d行胆管再通+肝动脉结扎组(C)、梗阻性黄疸5 d行胆管再通+部分门静脉动脉化组(D),每组10只。分别于术后1周应用HE染色观察胆管病理学改变,检测血清ALP、GGT变化,TUNEL染色观察胆管上皮细胞凋亡及坏死情况。结果 A组大鼠肝门部胆管病理学检查未见异常改变;B组大鼠肝门部胆管病理学检查见少量急慢性炎症细胞浸润,少部分胆管上皮细胞脱失;C组大鼠肝门部胆管可见大量急慢性炎症细胞浸润,胆管上皮细胞多量胞质空泡化改变,大量胆管上皮脱失,可见胆管壁坏死,胆管上皮细胞凋亡指数显著升高;D组大鼠肝门部胆管可见少量炎症细胞浸润,散在数个胆管上皮细胞空泡化,少部分上皮脱失,凋亡指数与B组比较无统计学差异。结论梗阻性黄疸情况下行肝动脉结扎会导致肝门部胆管严重的缺血缺氧改变,甚至胆管壁坏死,部分门静脉动脉化明显减轻肝总动脉结扎对梗阻性黄疸大鼠肝门部胆管的损害。Objective To study the effect of partial portal vein arterialization after bile duct flow reconstruction on hepatohilar bile duct, liver function and bile duct epithelium in rats with obstructive jaundice. Methods Forty rats were randomly divided into group A （control group）, group B undergoing bile duct recanalization 5 days after obstructive jaundice, group C undergoing bile duct recanalization ＋ hepatic artery ligation 5 days after obstructive jaundice, and group D undergoing bile duct recanalization ＋ partial portal vein arterialization 5 days after obstructive jaundice （10 in each group）. Pathological changes in bile duct were observed with HE staining, serum ALP and GGT levels were measured, and apoptosis and necrosis of bile duct epithelial cells were detected with TUNEL staining 1 week after operation. Results No distinct pathological change was observed in hepatohilar bile duct of group A. Filtration of a small number of inflammatory cells and loss of a small number of bile duct epithelial cells were found in group B. The following changes occurred in group C, including acute or chronic infiltration of inflammatory cells on hepatic bile duct wall, cytoplasm vacuolation of bile duct epithelial ceils, loss of massive bile duct epithelium, necrosis of bile duct wall, and significantly increased apoptosis index of bile duct epithelial cells. Infiltration of a small number of inflammatory cells, scattered vacuolation of bile duct epithelial cells and loss of some bile duct epithelium were detected in group D with no significant difference in apoptosis index and necrosis of bile duct wall. Conclusion Liver artery ligation leads to severe ischemic and anoxic change in hepatohilar bile duct and even necrosis of bile duct wall while partial portal vein arterialization can significantly alleviate the damage to hepatohilar bile duct caused by liver artery ligation in rats with obstructive jaundice.

关 键 词：门静脉动脉化 肝动脉结扎 梗阻性黄疸 胆管 大鼠 

分 类 号：R657.3[医药卫生—外科学]