干扰素α-1b对人眼Tenon囊成纤维细胞增殖抑制的实验研究  

Experimental Study of Inhibiting Effect of Interferon-alpha 1b on Proliferation of Human Tenon Capsule Fibroblasts

作　　者：黄楚龙[1] 蓝育青[1] 杨智宽[1] 林明楷[1] 

机构地区：[1]中山医科大学中山眼科中心,广州市510060

出　　处：《中国药房》2000年第6期253-254,共2页China Pharmacy

摘　　要：目的：观察国产干扰素α－１ｂ（赛若金）对人眼 Ｔｅｎｏｎ囊成纤维细胞增殖的抑制作用。方法：取人眼Ｔｅｎｏｎ囊成纤维细胞进行体外培养，传３代后用ＭＴＴ法检测干扰素α－１ｂ对人眼Ｔｅｎｏｎ囊成纤维细胞增殖的抑制作用。结果：干扰素α－１ｂ对人眼Ｔｅｎｏｎ囊成纤维细胞生长有明显的抑制作用，１０～６ＩＵ／ｍｌ作用组的ＯＤ值为（０．１１０９±０．０ ５８５）与对照组（０．２ ５３５±０．０ ５０２）之间有极显著性差异（Ｐ＜０．０１），作用组的抑制率为５６．２５％。结论：国产干扰素α－１ｂ对人眼Ｔｅｎｏｎ囊成纤维细胞增殖有明显的抑制作用。OBJECTIVE: To observe the inhibiting effect of Interferon-alpha 1b(IFNa-1b) on proliferation of human tenon capsule fibroblasts. METHODS: Human tenon capsule fibroblasts were cultured in vitro and MTT method was used to detect the inhibiting effect of IFNa-1b on human tenon capsule fibroblasts. RESULTS: There was significant difference in OD values between 10~6IU/ml group(0. 1 109±0. 0 585) and control group(0.2535±0. 0502), the inhibition rate in IFN group was 56.25%. CONCLUSION: IFNa-1b has significant effect on inhibiting proliferation of human tenon capsule fibroblasts.

关 键 词：干扰素Α-1B 成纤维细胞 MTT法 

分 类 号：R979.5[医药卫生—药品]