江南卷柏质量标准研究  

Study on Quality Standard of Selaginella moellendorffii

作　　者：刘义梅[1] 王柯[1] 陈科力[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065

出　　处：《中药材》2013年第9期1430-1434,共5页Journal of Chinese Medicinal Materials

基　　金：湖北省科技攻关计划项目(2006AA301B01)

摘　　要：目的:对江南卷柏药材进行生药鉴定研究,以制定其药材的质量标准。方法:观察江南卷柏药材的性状和显微鉴别特征;以穗花杉双黄酮为对照品进行TLC色谱研究;采用HPLC法建立江南卷柏药材的含量测定方法。结果:TLC法采用硅胶GF254板,以甲苯-甲酸乙酯-甲酸(5∶4∶0.5)为展开剂,FeCl3乙醇溶液为显色剂,可出现4个清晰的斑点;HPLC法以穗花杉双黄酮为对照品,以乙腈-水(含3%四氢呋喃和3%三氟醋酸)为流动相,检测波长330 nm,经对3批药材测定,药材中穗花杉双黄酮含量不少于0.4%。结论:江南卷柏药材的显微、TLC鉴别和穗花杉双黄酮的含量测定等方法准确可靠,可有效控制江南卷柏药材的质量。Objective： To study the quality standard of Selaginella moellendorffii. Methods： The macroscopic and microscopic characteristics were observed, amentoflavone was used as reference substance in the TLC identification and HPLC method was used to determine the content of amentoflavone. Results： The TLC method used GF254 taking toluene-ethyl formate-formic acid （5： 4： 0. 5 ）as the developer and ethanol solution of ferric chloride as the chromogenic reagent with the results that it appeared four clear spots. The HPLC method took amentoflavone as the reference substance,and acetonitrile-water （containing 3% tetrahydrofuran and 3% trifluoro- acetic acid）as the mobile phase;Detection wavelength was 330 nm. The contents of samples from 3 different batches were determined, and the lowest content of amentoflavone was 0.4%. Conclusion： This method is reliable and accurate ,which can be used for the quality control of SelagineUa moellendorfii.

关 键 词：江南卷柏 穗花杉双黄酮 HPLC含量测定 TLC鉴别 显微鉴别 

分 类 号：R282.5[医药卫生—中药学]