三种不同方法提取北细辛挥发油的成分分析  

Composition Analysis of Volatile Oil of Asarum heterotropoides var.mandshuricum Extracted by Three Different Methods

作　　者：陈燕銮[1] 刘秋琼[1] 王建壮[2] 陈楚萍[1] 王经韵[1] 

机构地区：[1]广东省人民医院/广东省医学科学院,广东广州510080 [2]广东药学院药科学院,广东广州510006

出　　处：《中药材》2013年第9期1447-1451,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨三种不同方法提取的北细辛挥发油成分的异同。方法:将北细辛用水蒸气蒸馏法、超临界二氧化碳萃取法提取的挥发油,超临界二氧化碳萃取的挥发油再减压浓缩除去挥发性成分,这三种方法得到的三组挥发油用气质联用方法进行成分分析。结果:水蒸气蒸馏法和超临界二氧化碳萃取法提取北细辛的挥发油有36个成分,其中相同的成分有16个,含量各不相同,超临界二氧化碳萃取后再提取法主要得到的是挥发油中的非挥发性成分,共鉴定出11种化学物质,主要为油脂类。结论:实验通过比较研究不同提取方法所得的北细辛挥发油成分,得出了它们之间的共同成分及相对含量,首次通过对超临界二氧化碳萃取法提取的挥发油中的不挥发性成分进行分析,为中药材北细辛的药用成分和质量控制提供了一定依据。Objective ： To study the differences of the constituents of the volatile oil from Asarum heterotropoides var. mandshuricum with three different extracted methods. Methods ： Volatile oil from Asarum heterotropoides var. mandshuricum was extracted by steam distillation, supercritical carbon dioxide extraction and supercritical carbon dioxide extraction followed by concentration under pressure to remove volatile components, respectivily. GC-MS was utilized to analyze the components of volatile oil. Results ： The volatile oil from Asarum heterotropoides var. mandshuricum extracted by steam distillation and supercritical carbon dioxide extraction method contained 36 ingredients, 16 of which were the same, but different in amount. 11 kinds of non-volatile components of the volatile oil from Asarum het- erotropoides var. mandshuricum were identified as the main components with supercrtical carbon dioxide extraction followed by concen- tration under reduced pressure ,which were mainly fats and oils. Conclusion ： The common components and their relative contents of the volatile oil from Asarum heterotropoides var. mandshuricum are obtained and compared with different extraction methods. It＇s the first time to extract the volatile components in the volatile oil from Asarum heterotropoides var. mandshurieum by supercritical carbon dioxide extraction, which provides the basis for Chinese herbal medicinal ingredients and quality control of the Asarum to some extent.

关 键 词：北细辛挥发油 提取 成分分析 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]