远志皂苷元神经营养作用研究  

Neurotrophic Effects of Senegenin

作　　者：皮婷[1] 薛小燕[2] 罗焕敏[3] 

机构地区：[1]昆明医科大学附属延安医院药学部,云南昆明650051 [2]赣州市人民医院药剂科,江西赣州341000 [3]暨南大学医学院药理学系,广东广州510632

出　　处：《中药材》2013年第9期1477-1480,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(30672450);广东省科技计划项目(2010B030700018)

摘　　要：目的:初步探讨远志皂苷元神经营养作用的机制。方法:体外培养新生大鼠皮质神经元,采用RT-PCR法对MAP2 mRNA及BDNF mRNA表达情况进行测定,进一步观察远志皂苷元的神经营养作用。结果:远志皂苷元低、中及高剂量均可明显提高细胞的活力,并呈一定的剂量依赖性;与溶媒对照组比较,远志皂苷元各剂量组MAP2mRNA及BDNF mRNA表达量均增加,以高剂量组(2μmol/L)最为显著(P<0.01)。结论:远志皂苷元能增加新生大鼠皮质神经元MAP2 mRNA及BDNF mRNA的表达,具有神经营养作用。Objective ： To study the neurotrophic effects of senegenin on the expression of MAP2 mRNA and BDNF mRNA in cultured cerebral cortical neurons. Methods：The newborn rat cerebral cortex neurons were cultured in vitro. LDH assay was used to investigate the effect of senegenin on the neuronal viability and reverse transcription polymerase chain reaction （RT-PCR）was carried out to determine the expression level of MAP2 mRNA and BDNF mRNA. Results ： LDH assay showed that senegenin at the concentration of 0. 5 μmol/L, 1 μmol/L and 2μmol/L could obviously enhance the survival of cells and the survival rates were in dose-dependent manner to some extent. Moreover, the low, medium and high concentrations of senegenin significantly increased the expression of MAP＇2 mRNA and BDNF mRNA. Conclusion ：The study suggests that suitable dose of senegenin can increase the expression of MAP2 mRNA and BDNF mRNA in cultured cerebral cortical neurons, and its mechanism needs further study.

关 键 词：远志皂苷元 大鼠皮质神经元 神经营养作用 

分 类 号：R285.5[医药卫生—中药学]