芦荟大黄素对高转移乳腺癌细胞MDA-MB-231侵袭与转移的影响  

Effect of Aloe Emodin on Invasion and Metastasis of High Metastatic Breast Cancer MDA-MB-231 Cells

作　　者：何振辉[1] 黄越群[1] 翁闪凡[1] 谭耀荣 何太平[2] 覃燕梅[2] 梁念慈[3] 

机构地区：[1]佛山科学技术学院医学院医学检验系,广东佛山528000 [2]广东医学院生物化学与分子生物学研究所,广东湛江524023 [3]广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《中药材》2013年第9期1481-1485,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(39870900);广东省中医药局建设中医药强省课题(20111057);佛山市医学类科技攻关项目(201108064);佛山科学技术学院博士启动基金(2012006);广东省大学生创新创业训练计划项目(1184713050)

摘　　要：目的:研究芦荟大黄素(Aloe emodin,AE)对人高转移乳腺癌细胞MDA-MB-231侵袭与转移的影响。方法:MTT法检测AE对MDA-MB-231细胞增殖的抑制作用;细胞-基质粘附试验检测AE对MDA-MB-231细胞粘附纤连蛋白(FN)、层连蛋白(LN)能力的影响;Transwell chamber法检测AE对MDA-MB-231细胞侵袭重组基底膜能力的影响;伤口愈合试验检测AE对MDA-MB-231细胞迁移能力的影响。裸小鼠模型观察AE对MDA-MB-231细胞实验性转移的影响。结果:80μmol/L AE能显著抑制MDA-MB-231细胞体外侵袭重组基底膜能力,显著抑制MDA-MB-231细胞粘附FN、LN的能力,其抑制率分别为(52.98±5.46)%,(34.99±2.63)%,(28.73±7.00)%。作用于MDA-MB-231细胞24 h后,AE能显著抑制MDA-MB-231细胞的迁移,80μmol/L AE处理的MDA-MB-231细胞在裸小鼠中形成肺转移结节的数量、体积明显减少。结论:AE抑制MDA-MB-231细胞的转移,其作用机制与抑制MDA-MB-231细胞的侵袭和迁移能力有关。Objective：To investigate the effect of Aloe emodin （AE）on the invasive and metastatic abilities of human high meta- static breast cancer MDA-MB-231 ceils. Methods：MTF assay was used to evaluate the viability of MDA-MB-231 ceils after treated with AE for 6 h and 24 h. The adhesive potential of MDA-MB-231 cells to FN and LN was tested by cell-matrix adhesion assay. The effect of AE on invasion of MDA-MB-231 cells was measured by Transwell chamber assay. Scratch wound healing assay was applied to determine the effect on migration of MDA-MB-231 ceils. The effect of AE on MDA-MB-231 lung metastasis was determined on an experimental metastatic model. Results： 80 μmol/L AE significantly inhibited the invasion, adhesion to FN, LN of MDA-MB-231 ceils in vitro, the inhibitory rates were （ 52. 98 ± 5.46 ） %, （ 34. 99 ± 2. 63 ） %, （ 28.73 ± 7.00 ） %, respectively. After 24 h treatment, AE significantly inhibited the migration of MDA-MB-231 cells. The number and volume of lung metastatic nodules formed by MDA-MB-231 cells after 80 μmol/L AE 24 h treatment were decreased compared with control group. Conclusion：AE can suppress the metastasis of MDA-MB-231 cells. Their mechanisms may be related to the inhibition of the capabilities of invasion and migration of MDA-MB-231 cells.

关 键 词：芦荟大黄素 粘附 侵袭 转移 乳腺癌 

分 类 号：R285.5[医药卫生—中药学]