健脾化湿汤对高尿酸血症模型大鼠的影响  

Effect of Jianpihuashi Decoction on Rats with Hyperuricemia

作　　者：陈健文[1] 周源[1] 薛照芸[1] 李承[1] 郭键[1] 周里岩 蒋建敏[1] 

机构地区：[1]中山大学药学院,广东广州510006

出　　处：《中药材》2013年第9期1486-1489,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨健脾化湿汤对高尿酸血症模型大鼠的影响。方法:SD大鼠40只,随机分成4组,每组10只,分别为:正常对照组、模型组、健脾化湿汤组和别嘌醇阳性对照组。于给药第0、10、20、30天取血,分离血清测定尿酸、肌酐、尿素氮和XOD活性。实验第30天,用3%戊巴比妥钠麻醉大鼠,取肝组织匀浆测定XOD活性,取肾切片、HE染色,光镜观察。结果:与模型组比较,健脾化湿汤给药30 d后可显著降低高尿酸模型大鼠血尿酸水平及血清、肝组织中XOD活性(P<0.05);通过血清BUN、Cr的检测结果和肾组织病理学检查结果显示,健脾化湿汤对高尿酸血症大鼠肾脏无显著的毒副作用;阳性对照药别嘌醇能显著降低尿酸(P<0.01),但加重了对肾脏的病理损害。结论:健脾化湿汤具有显著降低血尿酸的作用,作用机理可能与抑制黄嘌呤氧化酶的活性有关,且对肾脏无明显毒副作用。Ohjective：To investigate the effect of Jianpihuashi Decoction on rats with hyperurieemia. Methods：Forty male Sprague- Dawley（SD） rats were randomly divided into four groups：normal,hyperuricemia,Jianpihuashi Decoction and Allopurinol group. After the administration for 0 （lay, 10 （lays ,20 days and 30 days, the serum uric acid, crcatinine ,urea nitrogen and xanthine oxidase （XOD） activity levels were separately detected using the orbital blood. 30 days after the experiment,the rats were anaesthetized by 3% pentobarbital sodium, liver tissue homogenate extracts were used to detect the XOD activity,and histopathological changes in kidney were observed by HE staining. Results ：Treatment with Jianpihuashi Decoction for 30 days, the serum uric acid level of rats with hyperuricemia were significantly deereased（ P 〈 0. 05 ）. Simultaneously, the XOD activity in the serum and liver tissue homogenate extracts were obviously decreased by the decoction, which had seldom toxic or side effects on kidney. Allopurinol group could significantly decrease the serum uric aeid level, hut it had seldom pathological injury to kidney at the same time. Conclusion：Jianpihuashi Decoction which has seldom pathologieal injury, to kidney can significantly decrease the effect of uric acid by suppressing XOD activity.

关 键 词：健脾化湿汤 大鼠 尿酸 别嘌醇 

分 类 号：R285.5[医药卫生—中药学]