不育症患者睾丸曲细精管界膜的病理学观察  

Pathological observation on lamina propria of seminiferous tubules in infertile men

作　　者：孙辉臣[1] 董志英[1] 丁卫东[1] 王涛[2] 王更新[2] 曹玉纯 

机构地区：[1]白求恩国际和平医院男性生殖研究室,石家庄050082 [2]河北医科大学组织胚胎教研室

出　　处：《中国男科学杂志》2000年第4期243-246,共4页Chinese Journal of Andrology

基　　金：国家计划生育委员会资助项目

摘　　要：应用形态计量学和免疫组织化学方法观察 41例少精子症和无精子症患者的睾丸活检曲细精管界膜的病理学变化。曲细精管生精上皮的病理变化主要有三种类型 :生精功能低下、生精阻滞和唯支持细胞综合征。三种病变的曲细精管均表现有不同程度的界膜增厚。形态计量学结果显示三种病变的曲细精管界膜厚度、管内径与正常对照相比均有显著性差异 ,管内径与界膜厚度呈负相关。免疫组化层粘连蛋白及Ⅳ型胶原染色显示界膜的内外层呈阳性反应 ,而中间增厚带为阴性 ,Ⅰ型、Ⅲ型胶原免疫组化染色界膜增厚的中间带为阳性。提示 :病变界膜的成分为胶原纤维 ,但与正常成分———Ⅳ型胶原不同 。In this paper the authors observed the pathological alterations in lamina propria of seminiferous tubules from 41 patients with oligospermia and azoospermia by using morphometry and immunohistochemistry.Morphological alterations in seminiferous tubules of these patients were classified into three types:hypospermatogenesis,spermatogenic arrest and Sertoli cell only syndrome.The limiting membrane of the seminiferous tubules was found thicker in various degrees.Morphometrically,there were significant differences among the three types of alterations in terms of thickness of the lamina propria and the diameter of the seminiferous tubules.Immunohistochemically,the inner and outer layers of the lamina propria were positive for type Ⅳ collagen and laminin,while the intermediate layer,the hyperplastic zone,was negative,but this zone was positive for type Ⅰ and type Ⅲ collagen.The results indicated that the basic component of the altered lamina propria was of collagen fibers,but not of type Ⅳ collagen,and it consisted mainly of hyperplastic type Ⅰ and Ⅲ collagen fibers.

关 键 词：睾丸 男性不育 病理学 免疫组化 

分 类 号：R698.202[医药卫生—泌尿科学]