肺孢子肺炎大鼠模型及诊断方法的实验研究  

Experimental Study on Pneumocystic Pneumonia of Model of Rats and Diagnostic Method

作　　者：金长顺[1] 马和祥[1] 陈盛霞[2] 谈青芬 陈淼[3] 查小英[3] 仇锦波[2] 

机构地区：[1]镇江医学院实验动物中心,江苏镇江212001 [2]镇江医学院寄生虫学教研室,江苏镇江212001 [3]镇江医学院病理解剖学教研室,江苏镇江212001

出　　处：《镇江医学院学报》2000年第4期618-620,共3页Journal of Zhenjiang Medical College

摘　　要：目的 :为了便捷地建立肺孢子虫肺炎动物模型及优选其实验诊断方法。方法 :给大鼠皮下注射醋酸泼尼松龙 ,同时用全价颗粒饲料及加入四环素的饮水喂饲 ,建立卡氏肺孢子虫肺炎动物模型 ,以支气管肺泡灌洗物涂片、肺印片和肺组织病理切片进行实验诊断。结果 :从大鼠体内检测到卡氏肺孢子虫仅需 3周时间 ,与传统方法比较要提早 2～ 3周。支气管肺泡灌洗物涂片法阳性率为 94%。结论 :本研究不但饲喂方法简便 ,而且能较快地获取动物模型和原虫标本 ,故具有实用价值。支气管肺泡灌洗物涂片法可作为临床首选实验诊断方法。Objective: In order to establish animal model of pneumocystis carinii pneumonia conveniently. Methods: The animal model of pneumocystic pneumonia was established by injecting prednisolone acetate to SD rats and feeding them with acheomycin and drinking water contained tetracyclini.Using the smears of washing matter in bronchial vesicle and printed and pathologic seices of the lung make the experimental diagnosis. Results: Detecting pneumocystis carinii from the rats needs three weeks only.Earlier 2～3 weeks than the traditional method. The positive rate of pneumocystis carinii in washing liquid of lung was 94%. Conclusions: The study showed that this method is not only made conveniently but also has practical value in acquiring animal models and specimen of the protozoan. Detecting of washing liquid in the lung can be cho5en as the first method of diagnosis to pneumocystic pneumonia clinicaly.

关 键 词：卡氏肺孢子虫肺炎 醋酸泼尼松龙 实验诊断 

分 类 号：R563.104[医药卫生—呼吸系统]