外源λDNA导入普通小麦雄性不育变异的分子验证  

MOLECULAR VERIFICATION ON MALE STERILE MUTANT AFTER INJECTED EXOGENOUS λDNA INTO WHEAT

作　　者：杨景成[1] 于元杰[1] 刘凤珍[1] 齐延芳 沈法富[1] 

机构地区：[1]山东农业大学农学系植物遗传工程实验室,山东泰安271018

出　　处：《核农学报》2000年第6期371-374,共4页Journal of Nuclear Agricultural Sciences

摘　　要：将外源λDNA导入普通小麦品系 81 45 2 7,获得一细胞质雄性不育变异体 ,稳定成系 ,简称D型不育系 ,受体 81 45 2 7可作其保持系。用32 P标记的λDNA作为探针 ,对受体 81 45 2 7、D型不育系及其与鲁麦 1 4号的杂种F1 核DNA和叶绿体DNA进行点杂交。实验结果表明 :D型不育系及杂种F1 核DNA和叶绿体DNA点杂交均呈阳性反应 ,而受体无阳性反应。说明外源λDNA片段已经整合进了不育变异体核基因组和叶绿体基因组中 ,并且可以稳定遗传。A cytoplasmic male sterile mutant and then a stable cms line named D type sterile line were obtained after injected exogenous λDNA into wheat line 814527,and line 814527 could be its maintainer line.By using λDNA labled with 32 P as probe,unlabled λDNA as positive check,dot blottings of nuclear DNA and chloroplast DNA of receptor 814527,D type sterile line and its hybrid F 1 with Lumai 14 were carried out.Positive dots appeared in nuclear DNA and chloroplast DNA of D type sterile line and its hybrid F 1,but did not appear in the receptor.It showed that fragments of exogenous λDNA existed in nuclear genome and chloroplast genome of D type sterile line,and could be inherited stably.All these results,on a molecular level,proved the reliability of exogenous DNA injection.

关 键 词：外源DNA导入 小麦 细胞质雄性不育 点杂交 

分 类 号：Q943[生物学—植物学]