艾灸对大鼠胃黏膜损伤修复相关细胞信号蛋白质的影响  

Research on the Effect of Moxibustion on Gastric Mucosal Injury Repair Related Cell Signaling Proteins with Protein Fingerprinting

作　　者：杨宗保[1] 王晨光[2] 龚安[2] 谢宇锋 刘琼[2] 

机构地区：[1]厦门大学医学院 [2]江西中医药大学 [3]深圳市福田区中医院

出　　处：《中医杂志》2013年第22期1945-1948,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30960484;81260556);江西省自然(青年)科学基金资助项目(2008GZY0022);江西省教育厅2009年度科技计划资助项目(GJJ09280)

摘　　要：目的研究艾灸对胃黏膜损伤修复相关细胞信号蛋白质的影响,探讨艾灸促进胃黏膜损伤修复的分子机制。方法40只SD大鼠随机分为正常组、模型组、胃经穴组和对照点组各10只,采用束缚冷应激法建立应激性胃溃疡大鼠模型,胃经穴组大鼠造模后鼠板束缚,艾灸胃经"梁门"、"足三里"穴位;对照点组鼠板束缚,艾灸对照点;空白组和模型组仅鼠板束缚,各组均每次20min,每日1次,持续12天。应用蛋白质指纹图谱技术和疏水芯片、亲水芯片、金属离子螯合芯片检测各组大鼠胃黏膜细胞蛋白质指纹图谱,对比分析各组的差异蛋白质质荷比峰。结果各组蛋白质指纹图谱比较,3个芯片共检测出10个标志蛋白质差异表达,其中疏水芯片有3个标志蛋白质表达差异,分子量分别是2736Da、6985Da、7324Da;亲水芯片有1个标志蛋白质表达差异,分子量是4561Da;金属离子螯合芯片有6个标志蛋白质表达差异,分子量分别是1034Da、2136Da、3261Da、4871Da、7586Da、8267Da。结论艾灸可调节胃黏膜损伤模型大鼠多种相关信号蛋白质的差异表达,这可能是其促进胃黏膜损伤修复的分子机制之一。Objective To study the effect of moxibustion on gastric mucosal injury repair related cell signaling proteins and its molecular mechanism.Methods Forty SD rats were randomized into the normal（blank）group,model group,stomach channel point group and control point group,with 10in each.The stress ulcer rat models were established with restraint cold stress method.The stomach channel point group was given moxibustion on Liangmen（ST21）and Zusanli（ST36）.The control point group was given moxibustion on the control points.The rats in the blank and model groups were tied with mouse board only.Each group was given corresponding measures 20min each time and once daily for 12days.The mucosal cell protein fingerprint was detected with protein fingerprinting,hydrophobic chip,hydrophilic chip and metal ion chelating chip.The mass-to-charge ratio of differential protein in each group was compared and analyzed. Results Comparison of protein fingerprints in each group showed that 10signs of differentially expressed proteins were detected in 3chips.Three signs of differentially expressed proteins were detected in hydrophobic chip and their molecular weights were 2736Da,6985Da and7324Da.One sign of differentially expressed protein was detected in hydrophilic chip and its molecular weight was 4561Da.Six signs of differentially expressed proteins were detected in metal ion chelating chip and their molecular weights were 1034Da,2136Da,3261Da,4871Da,7586Da and 8267Da.Conclusion Moxibustion can regulate the differential expression in a variety of related signaling proteins and promote the repairation of gastric mucosal injury.

关 键 词：艾灸 蛋白质指纹图谱 胃黏膜 信号蛋白质 

分 类 号：R245[医药卫生—针灸推拿学]