UPLC同时测定酸枣仁中4种活性成分的含量  

UPLC Determination of Four Active Components in Ziziphi Spinosae Semen

作　　者：崔思娇[1] 罗洁[1] 张靓琦[1] 石绍淮[1] 毕开顺[2] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院,沈阳110016 [2]沈阳药科大学药学院,沈阳110016

出　　处：《世界科学技术-中医药现代化》2013年第6期1323-1328,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：辽宁省科学技术厅科学技术计划项目(2011412004):计算机辅助药物设计技术平台及产品开发;负责人:毕开顺;辽宁省教育厅创新团队项目(2009T097):酸枣仁汤质量控制与评价方法的研究;负责人:贾英

摘　　要：目的：建立UPLC同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B和白桦脂酸含量。方法：采用ACQUITY UPLC,HSS T3色谱柱（2.1 mm×100 mm,1.8μm）,流动相为乙腈-0.03%磷酸水溶液,进行梯度洗脱,流速0.5 mL·min-1,波长204 nm,进行检测。结果：斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B和白桦脂酸的质量浓度与峰面积分别在29.70~594.0μg·mL-1,10.68~213.6μg·mL-1,6.175~123.5μg·mL-1,22.00~440.0μg·mL-1范围内呈良好的线性关系。平均加样回收率均在98.3%~99.4%之间,RSD均小于1.4%。结论：该方法快速、准确、简便,为全面控制酸枣仁质量提供依据。This study was aimed to establish an analytical method to determine spinosin, jujuboside A, jujuboside B and betulinic acid content of Ziziphi Spinosae Semen quickly and accurately. The UPLC determination of Ziziphi Spinosae Semen method was established on an HSS T3 Column （2.1 mm × 100 mm, 1.8 μm） eluted with the mobile phase consisted of water containing 0.03% phosphoric acid and acetonitrile in gradient mode with the flow rate of 0.5 mL？min-1 and the detection wavelength was set at 204 nm. The standard curves of spinosin, jujuboside A, jujuboside B, betulinic acid showed a good linearity in 29.70 - 594.0 μg？mL-1, 10.68 - 213.6 μg？mL-1, 6.175 - 123.5 μg？mL-1, 22.00 - 440.0 μg？mL-1, respectively. The mean recoveries （n = 9） of the four components were between 98 . 3% and 99 . 4%, RSD 〈 1 . 4%. This method which is quick and accurate can be used to determine spinosin, jujuboside A, jujuboside B and betulinic acid in Ziziphi Spinosae Semen.

关 键 词：酸枣仁 斯皮诺素 酸枣仁皂苷A 酸枣仁皂苷B 白桦脂酸 UPLC 

分 类 号：R917[医药卫生—药物分析学]