不同品种石斛的HPLC-DAD-ELSD指纹图谱研究  

Study on HPLC-DAD-ELSD Fingerprint in Different Species of Dendrobii Caulis

作　　者：石媛慧[1] 郭力[1] 许莉[1] 郝普彦[1] 陈佳江[1] 韦练[1] 罗方利[1] 张廷模[1] 韩瑜 

机构地区：[1]成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137 [2]四川万安石斛产业开发有限公司,成都610213

出　　处：《世界科学技术-中医药现代化》2013年第6期1329-1332,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家"十二五"科技支撑计划项目(2011BAI13B02-8):石斛规范化种植基地及其SOP优化;升级研究;负责人:张廷模

摘　　要：目的:采用HPLC-DAD-ELSD建立石斛指纹图谱,并对金钗、鼓槌、叠鞘、细茎和反瓣等5种石斛的指纹图谱进行对比研究。方法:采用Dikma C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.5%冰乙酸水溶液,梯度洗脱,柱温30℃,流速1 mL·min-1,DAD串联蒸发光散射检测器检测。结果:建立5种石斛的HPLC指纹图谱,并标定9个指纹峰,5种石斛相似度较低。结论:建立的指纹图谱方法稳定、可靠、重现性好,可直接反映石斛品种与化学成分变化的关系。This study was aimed to set up the fingerprint chromatograms of chemical principles of Dendrobii Caulis by HPLC-DAD-ELSD. This analysis was performed at 30°C on a Dikma Cl8 column （250 mm × 4.6 mm, 5 μm） with a mobile phase consisting of acetonitrile-0.5% glacial acetic acid, gradient elution, at the flow rate of 1.0 mL？min-1 with DAD and ELSD detection. The HPLC fingerprint of Dendrobii Caulis was established. And 9 fingerprint peaks were marked. The similarity among different species was low. This method was stable, reliable and repeatable. It is useful for the study on the relationship between chemical components and species of Dendrobii Cauli.

关 键 词：石斛 指纹图谱 HPLC-DAD-ELSD 

分 类 号：R917[医药卫生—药物分析学]