酵母转化大黄结合型蒽醌研究  

Study on Conversion of Conjugated Anthraquinone in Radix et Rhizoma Rhei by Yeast Strain

作　　者：马超[1] 胡珊[1] 李学如[1] 张波[1] 孟涛[1] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《世界科学技术-中医药现代化》2013年第6期1333-1337,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：财政部教育部中央高校基本科研业务专项(SWJTU11ZT25):中药有效成分的生物转化;负责人:李学如

摘　　要：目的：从酵母菌中筛选出能将中药大黄结合型蒽醌转化为游离型蒽醌的菌株,为减轻生大黄的峻烈泻下作用提供一种选择。方法：通过考察培养基组分、种龄、发酵时间和装液量等因素对菌株转化中药大黄结合型蒽醌的影响。结果：筛选到菌株KM12,经26S rRNA分子鉴定为马克思科鲁维酵母（Kluyveromyces marxianus）。薄层层析表明,在酵母膏1.0%、葡萄糖2.0%、大黄2.0%的培养基中,按5%的接种量接种,培养36~48 h种子液到装有50 mL发酵培养基的250 mL三角瓶中,200 rpm摇床30℃发酵4d,可将中药大黄中具有峻烈泻下作用的结合型蒽醌大部分解或转化成游离型蒽醌。结论：可通过KM12菌株发酵法炮制大黄,使结合型蒽醌分解或者转化成游离型蒽醌,减轻大黄的峻烈泻下作用。A yeast strain KM12 , which can convert conjugated anthraquinone of rhub arb to free anthraquinone , was screened. And it is identified as Kluyveromyces marxianus by 26S rDNA. The percentage of free an-thraquinone in total anthraquinone of rhub arb as an indicator was used to investigate the effect of culture medium component and fermentation processing conditions, such as seed age, fermentation time and liquid volume on con-version of conjugated anthraquinone by KM12. The optimal fermentation medium was determined by orthogonal test L9（43） and the composition （%） is yeast extract 1, glucose 2, and rhubarb 2. The 5% （V/V） of the seed culture inoculated 36-48 h was inoculumed to fermentation medium in shake flask to ferment at 30℃, 200 r？min-1 for 4 days. TLC analysis showed most of conjugated anthraquinone decomposed or converted into free anthraquinone in fermented rhub arb . It was concluded that the side effect of severe diarrhea caused by Chinese medicine rhub arb can be alleviated through fermentation processing by KM12 strain .

关 键 词：结合型蒽醌 游离型蒽醌 大黄 马克思科鲁维酵母 薄层层析 

分 类 号：TQ460.38[化学工程—制药化工]