壮药白花九里明水煎液止血作用机制探讨  

Experimental Studies on Hemostatic Effect and Hemostatic Mechanism of Water Decoction of Blumea Megacephala (Randeria) Chang et Tseng

作　　者：黄艳[1] 宁小清[1] 原鲜玲[1] 钟霞军[1] 谈远锋[1] 丘芬[1] 林莹波 

机构地区：[1]广西中医药大学药学院,南宁530001

出　　处：《世界科学技术-中医药现代化》2013年第7期1552-1556,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广西壮族自治区中医药管理局面上项目(GZKZ10-045):白花九里明水提液止血作用和毒理学的研究;负责人:宁小清

摘　　要：目的:考察白花九里明水煎液的止血作用及其作用机制,并了解其对肝脏功能的影响。方法:玻片法、毛细管法测定小鼠凝血时间(CT),断尾法测定小鼠出血时间(BT),测定大鼠凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、血浆纤维蛋白原含量(FIB)、血小板计数(PLC)、血浆复钙时间(PRT)、谷丙转氨酶(ALT)、谷草转氨酶(AST)。结果:以白花九里明水煎液6.7 g·kg-1、13.4 g·kg-1、26.8 g·kg-1灌胃给药,可不同程度地缩短小鼠CT和BT,分给以4.7 g·kg-1、9.4 g·kg-1、18.9 g·kg-1灌胃给以大鼠,可对其PT、APTT、TT、PRT均有不同程度的影响;白花九里明水煎液一定剂量可降低肝脏功能指标ALT、AST。结论:白花九里明具有促凝血、止血作用,其作用机制可能是通过激活内、外源性凝血系统而止血;同时,对肝脏无明显损害。This study was aimed to evaluate the hemostatic effect and mechanism of action for water decoction of Blumea megacephala （Randeria） Chang et Tseng in order to understand its influence to the liver function. The glass slides method and capillary tube method were used in the measurement of the coagulation time （CT）. And the tail-cutting method was used to measure the bleeding time （BT）, prothrombin time （PT）, activated part clotting live en-zyme time （APTT）, thrombin time （TT）, content of plasma fibrinogen （FIB）, platelet count （PLC）, plasma complex cal-cium time （PRT）, alanine aminotransferase （ALT） and aspartate transaminase （AST）. The results showed that intragastric administration with different doses of water decoction of Blumea megacephala （Randeria） Chang et Tseng （6.7 g·kg-1, 13.4 g·kg-1, 26.8 g·kg-1） can reduce CT and BT of mice. And intragastric administration with different doses of wa-ter decoction of Blumea megacephala （Randeria） Chang et Tseng （4.7 g·kg-1, 9.4 g·kg-1, 18.9 g·kg-1） can produce different degrees of impact on PT, APTT, TT and PRT of rats. Certain dose of water decoction of Blumea megacepha-la （Randeria） Chang et Tseng can reduce ALT and AST. It was concluded that Blumea megacephala （Randeria） Chang et Tseng had the hemostatic effect and its mechanism of action may be through the activation of the intrinsic and extrinsic coagulation system. There was no obvious damage to the liver.

关 键 词：白花九里明 止血 作用机制 玻片法 毛细管法 断尾法 

分 类 号：R285.5[医药卫生—中药学]