百事乐胶囊对慢性应激抑郁模型大鼠行为活动及海马突触素表达的影响  

Effect of Baishile Capsule on Behavior and Expression of Hippocampal Synaptophysin in Chronic Stress and Depression Model Rats

作　　者：孟盼[1,2] 王宇红[1,2] 张秀丽[1,2] 蔡川[1,2] 韩远山[1] 

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南中医药大学/湖南省中药粉体与创新药物省部共建国家重点实验室,长沙410208

出　　处：《世界科学技术-中医药现代化》2013年第7期1562-1568,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：湖南省教育厅重点项目(11A087):复方抗抑郁中药百事乐胶囊调控抑郁模型大鼠海马神经元可塑性胞内信号转导机制的研究;负责人:王宇红

摘　　要：目的:探讨百事乐胶囊对慢性应激抑郁模型大鼠行为活动、学习记忆及海马CA3区突触素Ⅰ(SYNⅠ)和突触囊泡素(SYNA)表达的影响。方法:SD大鼠随机分为6组,即空白对照组、抑郁模型组、氟西汀组、百事乐胶囊(2.88 g·kg-1、1.44 g·kg-1、0.72 g·kg-1)组,采用慢性温和不可预见性应激加孤养的方式建立抑郁模型,造模同时灌胃给药,每日1次,连续给药21天,对照组、模型组等量蒸馏水灌胃,观察大鼠Open-field、1%蔗糖偏食度、水迷宫及体质量变化率的影响,免疫组化和原位杂交观察各组大鼠SYNⅠ和SYNA的表达变化。结果:行为学表明,百事乐高剂量治疗2周后可显著提升模型大鼠水平及垂直活动次数(P<0.01),百事乐中剂量治疗3周后可明显提升模型大鼠水平活动次数(P<0.05)。高剂量治疗第1周后、中剂量治疗3周后可明显提高模型大鼠的蔗糖偏食度(P<0.01或P<0.05)。高剂量治疗2周、中剂量治疗3周可明显提高模型大鼠的体质量变化率(P<0.01或P<0.05)。百事乐高、中剂量可以缩短模型大鼠的寻找并爬上平台的持续时间(EL),并能明显升高模型大鼠穿越目标象限的次数;同时高剂量可明显缩短目标象限潜伏时间(P<0.05)。免疫组化和原位杂交结果显示,与模型组比较,百事乐高剂量能明显促进模型大鼠海马CA3区SYNⅠ和SYNA的表达,中剂量可明显提升SYNA的表达(P<0.05或P<0.01)。结论:百事乐胶囊能改善抑郁模型大鼠的抑郁行为及海马CA3区SYNⅠ和SYNA的表达。This study was aimed to investigate Baishile Capsule on behavior, memory and expression of SYN I and SYNA in hippocampal CA3 region of depression model rats. SD rats were randomly divided into six groups, which were the control group, depression model group, fluoxetine hydrochloride group, and the Baishile Capsule group （2.88 g·kg-1, 1.44 g·kg-1, 0.72 g·kg-1）. The chronic stress depression model rats were established by chronic and mild unpredictable stressors as described in the literature. In the model group, medicine was intragastricly administered once per day and continued for 21 days. In the control group and model group, same volume of distilled water was intragastricly administered. The open-field test, preference for 1% sucrose solution, Morris water maze, and rate of weight were carried out and observed. Immunohistochemistry and in situ hybridization were used in the observation of the expression of SYN I and SYNA in each group of model rats. The results showed that high-dosage Baishile Capsule can significantly increase the horizontal and vertical activities of score after two-week treatment （P〈 0.01）. The middle-dosage Baishile Capsule can significantly increase the horizontal activity of score after three-week treatment （P〈 0.05）. The rat＇s preference for sucrose solution was obviously increased in the high-dosage group after one-week treatment and in the middle-dosage group after three-week treatment （P〈 0.01, or P〈 0.05）. The rate of weight of rats was obviously increased in the high-dosage group after two-week treatment and in the middle-dosage group after three-week treatment （P〈 0.01, or P〈 0.05）. The high-dosage and middle-dosage Baishile Capsule can shorten EL, and significantly increase the number of target quadrant. The high-dosage Baishile Capsule can obviously shorten the target quadrant latency （P〈 0.05）. Compared with the model group, the immunohistochemistry and in situ hybridization showed high-dosage Baishile can significantly promote the exp

关 键 词：百事乐胶囊 抑郁症 海马 突触素I 突触囊泡素 

分 类 号：R285.5[医药卫生—中药学]