尿锌的目视比色快速测定方法  

Rapid determination of zinc in urine by visual colorimetry

作　　者：王玉金[1] 蔡安 王玉珠 杜迅[1] 边倩茹 刘丽[1] 

机构地区：[1]河南省科学院生物研究所有限责任公司,郑州450008 [2]郑州兰森生物技术有限公司,郑州450000

出　　处：《中国卫生检验杂志》2013年第9期2064-2066,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立一种以目视比色法半定量测定人尿液中锌含量的快速测定方法。方法在表面活性剂存在下,锌与硫氰酸盐和结晶紫形成三元离子缔合物,该胶束增溶体系随三元缔合物胶束的浓度不同而显示不同的颜色。结果经测定36份样本,与吡啶偶氮萘酚(PAN)显色法测定结果比较,两结果的符合率为97.22%。结论该方法简便、快速、结果准确直观,可用于尿液锌含量的半定量快速测定。Objective To establish a rapid method for semiquantitative determination of zinc in human urine by visual colorimetry.Methods In the presence of surfactant,zinc reacts with thiocyanate and crystal violet to form a ternary ion-association complex,which can show different colors with the concentration change.Results This method was compared with routine pyridylazo naphthol method（PAN） by testing 36 urine samples and the coincidence rate was 97.22%.Conclusion The method is simple,rapid,accurate and intuitive,which can be used for semiquantitative determination of zinc content in urine.

关 键 词：锌 硫氰酸钠 结晶紫 目视比色法 

分 类 号：R446.12[医药卫生—诊断学]