青岛市HIV抗体初筛阳性标本的复检及确证结果分析  

Rescreening and confirmation of anti-HIV antibody positive serum in primary screening in Qingdao

作　　者：史晓燕[1] 张洪花[1] 赵国有[1] 弋英[1] 汪照国[1] 

机构地区：[1]山东省青岛市疾病预防控制中心,山东青岛266033

出　　处：《中国卫生检验杂志》2013年第9期2117-2119,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的复检和确认HIV抗体初筛阳性血清并分析相关资料,为青岛市艾滋病防治工作提供依据。方法对初筛阳性血清,用胶体硒和ELISA试验复检,任一复检试验阳性的血清再用WB试验确证并对确证阳性病例进行流行病学分析。结果 1348份初筛阳性血清经复检745份阳性,WB确证563份HIV-1抗体阳性,不确定95份,阴性87份。ELISA、胶体硒法与WB的阳性符合率分别为81.5%和84.3%,二者的敏感度均为100%,特异度分别为93.6%和94.5%,确证阳性人群中,外来人员占58.4%,男男性行为人群占40.1%。结论 ELISA和胶体硒法用于HIV复检可排除大部分初筛假阳性,但两种复检试验均存在一定的假阳性。外来人员和男男性行为人群为青岛市HIV的主要感染人群,需加强干预。Objective To rescreen and confirm anti-HIV antibody positive serum in primary screening in Qingdao from 2009 to 2011 and to analyze the related data,so as to provide basis for prevention and control of AIDS in Qingdao.Methods Samples positive for anti-HIV antibody in primary screening test were re-screened with both Electroselenium method and ELISA.Then the positive samples in either re-screening test were further confirmed with Western Blot（WB）.Results In 1348 positive samples from primary screening test,positive were found in 745 cases in re-screening test.By WB confirmation,563 cases were positive,95 cases were suspected and 87 cases were negative.In contrast to WB,positive coincidence rates of ELISA and Electroselenium method were 81.5% and 84.3% respectively.The sensitivity and specificity of ELISA were 100% and 93.6 % respectively,and those of Electroselenium method were 100% and 94.5% respectively.Flouting population accounted for 58.4% and MSM accounted for 40.1% of total HIV infection.Conclusion Anti-HIV antibody rescreening tests could exclude most false positive samples in primary screening test.However,there is still some false positive rate in either rescreening test.Flouting population and MSM are the high risk population of HIV infection in Qingdao city and intervention should be strengthened among those people.

关 键 词：HIV 复检 确证 

分 类 号：R512.91[医药卫生—内科学]