CTX-M基因在福建省多重耐药志贺菌中分布研究  

Study on the distribution of CTX-M gene in the multidrug resistant Shigella in Fujian

作　　者：杨劲松[1,2] 陈爱平[1,2] 黄柳青[1] 罗朝晨[1] 王灵岚[1] 严延生[1,2] 

机构地区：[1]福建省疾病预防控制中心,福建福州350001 [2]福建省人兽共患病研究重点实验室,福建福州350001

出　　处：《中国卫生检验杂志》2013年第13期2714-2717,共4页Chinese Journal of Health Laboratory Technology

基　　金：福建省医学创新课题(2011-CXB-19)

摘　　要：目的了解福建省多重耐药志贺菌CTX-M基因及基因家族中各耐药基因组的分布情况;结合耐药谱分析该类基因和多重耐药的关系。方法煮沸裂解法提取2005年-2010年福建省细菌性痢疾监测分离到的103株志贺菌的DNA,PCR检测CTX-M基因以及亚群基因组(CTX-M-I、II、III、IV、V)。结果 CTX-M及亚群基因阳性率分别为33.98%、6.8%、20.39%、0%、20.39%、0%;福氏和宋内氏志贺菌CTX-M及亚群基因阳性率差别无统计学意义。AMP、AMC、CFT、CEF、CTX及CIP耐药组与非耐药组CTX-M基因分布存在差异,P<0.05。菌株同时携带CTX-M-Ⅱ和Ⅳ基因组为优势基因模式。结论 CTX-M基因与志贺菌β-内酰胺类抗生素耐药存在关联;福建省多重耐药志贺菌CTX-M的亚群以CTX-M-Ⅱ和Ⅳ常见;且同时携带这两种基因组,可能是菌株对β-内酰胺类抗生素耐药的优势基因模式。Objective To understand the distribution characteristics of CTX-M genes of multi-drug resistant Shigella in Fujian province,then analyze the relationship between these genes and multi-drug resistant phenomenon.Methods One hundred and three strains of Shigella collected from the monitoring program during 2005-2010 in Fujian province were boiled to extract DNA,and CTX-M genes and sub-group genomes（CTX-M-I,II,III,IV,V） were detected by polymerase chain reaction.Results The positive rates of CTX-M,CTX-M-I,II,III,IV,V were 33.98%,6.8%,20.39%,0%,20.39%,0%,respectively.There were no statistical differences of CTX-M and subgroup genome positive rates between Shigella flexneri and Shigella sonnei.There were statistical differences between drug-resistant group and non-resistant group classified by the drug resistant results of Ampicillin,Amoxicillin,Cefalotin,Cefaclor and Cefotaxime,P0.05.The gene pattern of the strains carrying CTX-M-Ⅱ and CTX-M-Ⅳ was the prevalent pattern.Conclusion There were relationship between CTX-M gene and drug resistance of β-lactam antibiotics.CTX-M genes were common genes of the multi-drugss resistant Shigella in Fujian province,and Shigella strains carrying CTX-M-Ⅱ and CTX-M-Ⅳ simultaneously may be the prevalent gene pattern of β-lactam drug resistance.

关 键 词：志贺菌 CTX-M基因 Β-内酰胺类抗生素 PCR 

分 类 号：R378.25[医药卫生—病原生物学]