淋巴细胞的KIR2DL4基因表达在反复性胚胎停育主动免疫治疗前后的研究  

Study of expression levels of KIR2DL4 gene in lymphocytes in repeated embryo loss before and after the active lymphocytes immunotherapy

作　　者：张怡梅[1] 高建军[1] 齐峰[1] 孙明芳[1] 丁丽萍[1] 

机构地区：[1]山东省潍坊市妇幼保健院生殖健康科,山东潍坊261011

出　　处：《中国优生与遗传杂志》2013年第11期21-23,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的检测复发性胚胎停育患者淋巴细胞主动免疫前后KIR2DL4基因的表达的变化,探讨反复性胚胎停育免疫学原因。方法选择2011年6月至2012年6月就诊的2次以上早孕胚胎停育的患者,根据以往有无人工流产和/或者分娩史分为无妊娠史(无人工流产及分娩史)组30例;有妊娠史(有人工流产史及分娩史)组55例;对照组为正常无妊娠史的未孕妇女30例。对研究者进行淋巴细胞主动免疫治疗4个周期后,采用荧光实时定量PCR技术检测治疗前后患者的淋巴细胞的KIR2DL4基因的表达。随访研究者妊娠结局。结果 (1)两组胚胎停育组治疗前KIR2DL4基因表达无妊娠史组为(43.55±12.71×103/ml)、有妊娠史组为(21.48±9.43×103/ml),分别与对照组(55.35±12.68×103/ml)比较,差异有统计学意义(P<0.05);两组胚胎停育组经淋巴细胞主动免疫治疗后KIR2DL4基因的表达分别为(56.76±10.90×103/ml、67.89±11.39×103/ml)与对照组治疗后(70.84±13.47×103/ml)比较,差异无统计学意义(P>0.05)。两组胚胎停育组免疫治疗前后比较KIR2DL4基因比较差异有统计学意义(P<0.05)。(2)免疫治疗前有妊娠史胚胎停育组KIR2DL4基因的表达(21.48±9.43×103/ml),与无妊娠史胚胎停育组KIR2DL4基因表达(43.55±12.71×103/ml)比较,差异有统计学意义(P<0.05),主动免疫治疗后差异无统计学意义(P>0.05)。(3)随访结果:两组胚胎停育组免疫治疗结束后,每组妊娠患者中,胚胎停育率分别为21.74%,18.75%;对照组胚胎停育率为20%。胚胎停育率比较差异无统计学意义(P>0.05)。结论淋巴细胞KIR2DL4基因的表达数量异常与反复性胚胎停育有关。Objective ： To analyze the changes of expression levels of KIR2DL4 gene in lymphocyte in repeated embryo loss before and after the active lymphocytes immunotherapy in women, in order to disscuss the immunologic mechanism of repeated embryo loss. Methods： The patients with more than twice embryo loss were chosen in the clinic from June 2011 to June 2012. According to if they had induced abortions and/or delivery histories, they were devided into two groups, one with no pregnancy histories has 30 cases, the other with pregnancy histories has 55 cases. The controle group was those normmal married women with no abortion and delivery histo- ry. By using the real - time quantitative PCR technique, the expression levels of KIR2DL4 gene was detected both of the two embryo loss groups before and after carried out the avtive lymphcytes immunotherapy. Then the results were compared with the control group. Result： 1. Before the active lymphocytes immunotherapy, the expression levels of KIR2DL4 gene in the two embryo loss groups （43.55 ~ 12. 71 x lO3/ml）, （21.48 ~9. 43 x 103/ml） were significantly lower than the control group （55.35 ：t： 12. 68 x 103/ml） ; After the active lymphocytes immunotherapy, the expression levels of the two embryo loss groups （56. 76 ~ lO. 90 x 103/ml）, （67. 89 ＋ 11.39 x 103/ml） were no statistically significant compared with the control group （70. 84 ~ 13.47 x 103/ml）. 2. The differences of the expression levels between the two embryo loss groups were statistically significant before the active immuotherapy, and no statistically significant after the active therapy. 3. Following - up results The embryo loss rates in those groups were 21.74% in no pregnancy history group, 18.75% in pregnancy histories group, and 20% in control group, respectively. There were no statistically significant compared by each of them. Conclusion： The abnormal expression levels in embryo loss cases in lymphcytes are related to the repeated embryo loss.

关 键 词：反复性胚胎停育 KIR2DIA-基因 淋巴细胞 

分 类 号：R714.21[医药卫生—妇产科学]