一测多评法测定枇杷叶有效部位中6种三萜酸成分的量  

Determination of six triterpene acid contents in effective fraction of Eriobotryae Folium by quantitative analysis of multi-components by single-marker

作　　者：蔡雪萍[1,2] 李振华[1,2] 华俊磊[1,2] 鞠建明[2] 吕寒[3] 马丽[3] 李维林[3] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]江苏省中医药研究院,江苏南京210028 [3]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《中草药》2013年第21期3057-3062,共6页Chinese Traditional and Herbal Drugs

基　　金：江苏省产学研前瞻性联合研究项目(BY2009144)

摘　　要：目的建立枇杷叶有效部位中蔷薇酸、委陵菜酸、马斯里酸、科罗索酸、齐墩果酸和熊果酸的一测多评HPLC-ELSD测定方法。方法以熊果酸对照品为内参物,建立其与蔷薇酸、委陵菜酸、马斯里酸、科罗索酸、齐墩果酸的相对校正因子,采用校正因子计算这5种三萜酸成分的量,实现一测多评;同时用外标法测定有效部位中该6种成分的量,并比较计算值与实测值的差异,以验证一测多评的合理性、可行性和重复性。结果 15批有效部位中6种三萜酸成分量的计算值与实测值无显著性差异。结论该方法操作简便,测定结果准确,实现了只用一个对照品同步测定枇杷叶总三萜酸有效部位中6个成分的量,可为中药多指标成分质量评价模式提供参考。Objective To develop a quantitative analysis of multi-components by single-marker （QAMS） method for the simultaneous determination of six triterpene acid （euscaphic acid, tormentic acid, maslinic acid, corosolic acid, oleanolic acid, and ursolic acid） contents in the effective fraction of Eriobotryae Folium. Methods Ursolic acid was used as the internal reference substance, and the relative correlation factors （RCF） of euscaphic acid, tormentic acid, maslinic acid, corosolic acid, and oleanolic acid were determined by HPLC-ELSD with good reproducibility. The contents of the five components were calculated according to the RCF, respectively. The contents of these six triterpene acids in 15 batches of effective fraction were determined by the external standard method. The rationality, feasibility, and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Results For the six triterpene acids, there was no significant difference between the quantitative results with the two different methods in the 15 batches. Conclusion The method established in this research is accurate and feasible that it just needs to assay single-marker （ursolic acid） for the determination of six triterpene acids in the effective fraction ofEriobotryae Folium simultaneously. Therefore, this method could provide a new reference for the quality assess of multi components in Chinese materia medica.

关 键 词：一测多评 枇杷叶 蔷薇酸 委陵菜酸 马斯里酸 科罗索酸 齐墩果酸 熊果酸 

分 类 号：R286.022[医药卫生—中药学]