虚拟模板分子印迹固相萃取-高效液相色谱法检测果蔬中三唑类杀菌剂  

Detection of triazole fungicides in fruit and vegetable by virtual-template molecularly imprinted solid phase extraction-high performance liquid chromatography

作　　者：刘博[1] 尹航[1] 高文惠[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《河北科技大学学报》2013年第5期417-421,共5页Journal of Hebei University of Science and Technology

基　　金：河北省科技支撑计划(10276902D);石家庄市科技支撑计划(12149472A)

摘　　要：采用本体聚合法自制了联苯三唑醇分子印迹聚合物（MIP）,以此聚合物为固相萃取剂,制备固相萃取柱,对果蔬中烯唑醇、戊唑醇进行分离富集,并采用高效液相色谱法测定其在果蔬中的残留。烯唑醇平均回收率在74.6%~88.6%之间,检测限为0.5μg/mL。戊唑醇平均回收率在66.9%~73.3%之间,检测限为0.25μg/mL。The solid phase extraction column is prepared by mass polymerization with bitertanol molecularly imprinted polymer（MIP）as solid phase extraction agent.Diniconazole and tebuconazole in fruit and vegetable can be separated and enriched by the column,and their residuals are determined by high performance liquid chromatography（HPLC）.The average recoveries of diniconazole and tebuconazole are 74.6%~88.6% and 66.9%~73.3%,respectively.The detection limits are 0.5μg/mL for diniconazole and 0.25μg/mL for tebuconazole.

关 键 词：三唑类杀菌剂 虚拟模板分子印迹聚合物 固相萃取 高效液相色谱法 

分 类 号：O657.32[理学—分析化学] O641.3[理学—化学]