铜绿微囊藻叶绿素荧光对HgCl_2毒性响应特性的研究  

Study on the characteristics of Microcystis aeruginosa chlorophyll fluorescence responding on the toxicity of HgCl_2

作　　者：徐小惠[1,2] 段莉丽[1,2] 崔建升[1,2] 

机构地区：[1]河北科技大学环境科学与工程学院,河北石家庄050018 [2]河北省污染防治生物技术实验室,河北石家庄050018

出　　处：《河北科技大学学报》2013年第5期477-481,共5页Journal of Hebei University of Science and Technology

基　　金：国家863计划项目(2007AA092201)

摘　　要：利用叶绿素荧光技术,以铜绿微囊藻在435nm/680nm处的荧光强度为测试指标,进行了铜绿微囊藻叶绿素荧光对Hg2＋毒性的最佳响应时间,以及不同Hg2＋浓度在短时间内对铜绿微囊藻叶绿素荧光强度的影响研究。研究结果表明,铜绿微囊藻对HgCl2的最佳响应时间为25min。当Hg2＋质量浓度为0.000 5mg/L时,铜绿微囊藻的相对荧光强度（样品荧光强度-空白荧光强度）为负值,即其叶绿素荧光强度小于对照组的叶绿素荧光强度;当Hg2＋质量浓度为0.001~0.500mg/L时,铜绿微囊藻的相对荧光强度为正值,并且在0.001~0.400mg/L浓度范围内,相对荧光强度随汞浓度的增大而增大,其呈正相关关系,r=0.983 3。The toxicity of heavy metal Hg2＋ on alga Microcysis aeruginosa was studied by the change in fluorescence intensity of Microcysis aeruginosa at 435nm/680nm which treated with different Hg2＋ concentrations for 25 min.When the Hg2＋ concentration is 0.000 5mg/L,the relative fluorescence intensity of Microcystis aeruginosa（the sample fluorescence intensity minus the blank fluorescence intensity is equal to the relative fluorescence intensity）is negative;while when the Hg2＋ concentration is 0.001~0.500 mg/L,the relative fluorescence intensity of Microcystis aeruginosa is positive.And the relative fluorescence intensity increases with the concentration of Hg2＋ （0.001~0.400mg/L）,even the concentration of Hg2＋ and algal photosynthetic signal have a significant positive correlation（r=0.983 3）.

关 键 词：叶绿素荧光 铜绿微囊藻 Hg2+ 生物毒性 

分 类 号：X832[环境科学与工程—环境工程]