黄瓜香水提物对自由基损伤心肌细胞的作用  

Experimental Study on the Influence of Viola Diffusa Ging Extract on Myocardial Cells

作　　者：何轩[1] 彭光勇[1] 黄露露[1] 王云召[1] 刘锦[1] 彭湘萍[1] 李春艳[1] 

机构地区：[1]吉首大学医学院,湖南吉首416000

出　　处：《吉首大学学报（自然科学版）》2013年第3期66-68,共3页Journal of Jishou University(Natural Sciences Edition)

基　　金：湖南省大学生创新性学习和创新性实验计划资助项目(湘教通(2011)272号);吉首大学大学生研究性学习和创新性实验资助项目(JSU-CX-2011-36)

摘　　要：目的观察黄瓜香水提物对自由基损伤的心肌细胞的作用.方法体外培养心肌细胞,以不同浓度的黄瓜香水提物进行干预,测定心肌细胞搏动频率及培养液中乳酸脱氢酶(LDH)的活性,采用MTT法观察黄瓜香水提物对心肌细胞存活率的影响.结果自由基对心肌细胞造成损伤,不同浓度的黄瓜香水提物可减少LDH漏出,增加细胞存活率(P<0.05).结论黄瓜香对自由基损伤的心肌细胞具有保护作用.Objective： to study the influence of viola diffusa gine（VDG） extract on myocardial cells.Methods：myocardial cells were cultured with different concentrations of VDG extract to intervene.The myocardial cells pulse frequency and the contents of lactate dehydrgenase（LDH） in culture solution were determined.MTT method was used to observe the cells survival rate.Results：free radicals injured myocardial cell,VDG extract reduced LDH leakage and increased the cell survival rate（P0.05）.Conclusion：The results indicate that VDG extract has a certain protective effects on myocardial cells injured by free radicals.

关 键 词：黄瓜香水提物 心肌细胞 自由基 

分 类 号：R285.5[医药卫生—中药学]