氧化低密度脂蛋白致损人单核细胞白血病细胞株源性巨噬细胞Notch信号的表达  

The effect of oxidized low-density lipoprotein on Notch signal expression in macrophages

作　　者：付文波[1] 李斌[2] 龚志刚[1] 陈志楠[1] 蒋桔泉[1] 卢青[1] 彭毅[1] 王华[1] 丁世芳[1] 

机构地区：[1]广州军区武汉总医院心内科,湖北武汉430070 [2]湖北省松滋市人民医院心内科,湖北松滋434200

出　　处：《中国心脏起搏与心电生理杂志》2013年第5期427-430,共4页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：湖北省自然科学基金(No.2012FFB06803);湖北省卫生厅青年人才项目(No.QJX2012-24)资助

摘　　要：目的研究氧化型低密度脂蛋白(ox-LDL)致损人单核细胞白血病细胞株(THP1)源巨噬细胞Notch信号的表达,探讨Notch信号各成分对动脉粥样硬化(AS)发病的可能作用。方法用不同浓度的ox-LDL刺激THP1源巨噬细胞,在不同时间分别应用实时定量聚合酶链式反应及蛋白免疫印迹检测Notch信号通路受体配体的差异表达。结果与对照组比较,加入ox-LDL后,THP1源巨噬细胞四种受体中Notch1表达明显升高(P<0.05),五种配体中DlL4和Jagged1表达明显升高(P<0.05);Notch1、DlL4和Jagged1升高的作用在ox-LDL浓度为50 mg/L 6 h点作用最强。结论 ox-LDL致损THP1源巨噬细胞参与AS的发生与Notch1,DlL4和Jagged1高表达有关。Objective To explore the expression of Notch signal by oxidized low-density lipoprotein（ox-LDL） in mac- rophages of human monocytic leukemia cell line（ THP1 ） and to search for possible mechanism of atherosclerosis （ AS ）. Methods Human macrophage from THP1 cell line transform by PMA was cultured with different concentration of ox-LDL at different time to induce proinflammatory response. The expression of four receptors and five ligands of Notch mRNA and protein were measured by RT-PCR and Western blot respectively. Results The compared with control group, with the joining of ox-LDL, Notch 1 expression in four receptors of macrophages increased significantly（P〈0.05）, DIL 4 and Jag- ged 1 expression in five ligands increased significantly（P〈0.05 ） ; The expression of Notch 1, DIL 4 and Jagged 1 signifi- cantly increased in ox-LDL concentration of 50 mg/L at 6 h point which was strongest. Conclusion Notchl ,DIIA,Jag- gedl were activated by ox-LDL stimulation and may partially mediate atherogenic effect macrophage functions.

关 键 词：心血管病学 氧化型低密度脂蛋白 NOTCH信号通路 人单核细胞白血病细胞株 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]