蛇床子素在大鼠肠缺血再灌注肺损伤模型中的肺保护机制研究  

Lung Protection of Osthole Against Intestinal Ischemia-Reperfusion Lung Injury in Rats

作　　者：葛鹏[1,2] 杨波[1] 李小飞[1] 周琳[1] 景瑞军[2] 李汉杰[2] 姜涛[1] 

机构地区：[1]第四军医大学附属唐都医院胸腔外科,陕西西安710038 [2]西安医学院第二附属医院心胸外科,陕西西安710038

出　　处：《现代生物医学进展》2013年第28期5401-5404,5417,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81070062)

摘　　要：目的:探讨蛇床子素(Osthole)在大鼠肠缺血再灌注肺损伤(IIRI)中的保护机制。方法:健康雄性SD大鼠40只随机分为假手术组(sham组)、缺血再灌注组(I/R)和两个不同剂量给药组(Ost),其中缺血再灌注组和给药组采用夹闭大鼠肠系膜上动脉60 min后松开动脉夹形成缺血再灌注模型。各给药组于缺血再灌注30min后分别给予5 mg/kg、25 mg/kg蛇床子素腹腔注射治疗。各实验组动物分别采用HE染色、肺组织水肿(W/D)比值、ELISA检测和免疫组化评价蛇床子素在缺血再灌注肺损伤后的肺保护机制。结果:缺血再灌注30 min后,给药组大鼠HE,W/D、IL-1β和TNF-α结果均明显优于对照组(P<0.05),并在25 mg/kg剂量时表现出最大保护效果,同时Caspase-3蛋白表达水平也显著降低。结论:蛇床子素在大鼠肠缺血再灌注肺损伤后有一定的肺保护作用。Objective： To investigate the lung protection of osthole against intestinal ischemia-reperfusion lung injury in rats. Methods： A total of 40 SD rats were divided randomly into 4 groups： sham operated group （Sham）; I/R group （I/R）; Osthole 5 mg / kg group （Ost5） and osthole 25 mg / kg group （Ost25）. The animals in I/R group and osthole groups were exposed to the IIRI rat model. After 60 min of occlusion and 30min ofreperfusion, the rats in osthole groups were treated with 5 mg / kg, and 25 mg / kg osthole respectively. 30 min after the reperfusion, the pathological changes by hematoxylin and eosin （HE） dyeing were observed microscopically, wet, dry （W/D） ratio, IL-1β and TNF-α of lung tissues were examined for every groups. The expression of Caspase-3 protein in the lung tissue of every group was detected by immunohistochemistry. Osthole groups were better than that of I/R group （P〈0.05）. Results： Both the histopathologieal changes, W/D, IL-1β and TNF-α ofosthole groups were better than that of I/R group （P〈0.05）. Osthole could reduce the elevated level of caspase-3 protein induced by the ischemia and reperfusion. Conclusion： Osthole exerts protective effect against intestinal ischemia-reperfusion lung injury in rats.

关 键 词：蛇床子素 肠缺血再灌注肺损伤 半胱氨酸依赖的天冬氨酸蛋白酶-3 肺保护 

分 类 号：Q95-3[生物学—动物学] R655.3[医药卫生—外科学]