缺血后处理对老年大鼠再灌注心肌的保护作用及其与P-Akt的相关性  

Ischemic Postconditioning Protects Elderly Rat Hearts against Ischemia-reperfusion Injury via P-Akt Signaling Pathway

作　　者：王波[1] 陶凉[1] 陈绪发[1] 冯学国[1] 

机构地区：[1]武汉亚洲心脏病医院心脏外科,武汉430022

出　　处：《中国胸心血管外科临床杂志》2013年第6期710-713,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目的 探讨缺血后处理对老年大鼠离体心脏缺血-再灌注损伤的影响及其与P-Akt的相关性。 方法21～23个月龄 （老年鼠） 健康SD大鼠30只，雌雄不拘，体重450～500 g，分为空白对照组（N组）、缺血-再灌注组（IR组）和缺血后处理组 （Post组） 3组，每组10只，观测心脏冠状动脉流量、心肌梗死范围、磷酸化的蛋白激酶B （P-Akt）表达、心肌和线粒体的改变。 结果 Post组较IR组冠状动脉流量明显增加 〔（6.4±1.2） ml/min vs. （3.1±1.2） ml/min，P＜0. 01〕，心肌梗死范围明显减少（35.0%±2.0% vs. 55.7%±3.6%），P-Akt的表达明显增强，心肌纤维和线粒体的完整程度明显较好。 结论 缺血后处理对老年大鼠离体心脏具有显著的保护作用，这在一定程度与P-Akt激活有关。Objective To explore the impact of ischemic postconditioning on ischemia-reperfusion injury in isolatedelderly rat hearts and their relation with P-Akt. Methods A total of 30 healthy elderly SD rats （21-23 months old， male or female） with their body weight of 450-500 g were divided into 3 groups： control group， ischemia-reperfusion group， and postconditioning group， with 10 rats in each group. Coronary artery blood flow，myocardial infarction size， phosphorylatedAkt （p-Akt） expression， and changes in myocardium and mitochondria were detected. Results Coronary artery blood flow of the postconditioning group was significantly higher than that of the ischemia-reperfusion group （6.4±1.2 ml/min vs.3.1±1.2 ml/min， P＜0. 01）， and myocardial infarction size of the postconditioning group was significantly smaller thanthat of the ischemia-reperfusion group （35.0%±2.0% vs. 55.7%±3.6%， P〈0. 05）. The expression of P-Akt was significantlyhigher， and myocardial fibers and mitochondria were preserved better in the postconditioning group than the ischemia-reperfusion group. Conclusion Ischemic postconditioning can protect isolated elderly rat hearts against ischemia-reperfusion injury， which may be related to P-Akt activation.

关 键 词：缺血后处理 心肌保护 缺血-再灌注 P-AKT 

分 类 号：R541[医药卫生—心血管疾病]