落叶松—杨栅锈菌遗传分化的RAPD分析  

ANALYSIS OF THE GENETIC SPECIALIZATION OF \%MELAMPSORA \% LARICI POPULINA WITH RAPD MARKERS

作　　者：田呈明[1] 康振生[1] 李振岐[1] 赵彦修[2] 张慧[2] 

机构地区：[1]西北农林科技大学,杨陵712100 [2]山东师范大学,济南250014

出　　处：《林业科学》2000年第5期54-58,共5页Scientia Silvae Sinicae

基　　金：西北农林科技大学青年基金;山东师范大学逆境植物重点实验室资助

摘　　要：用随机扩增多态性DNA(RandomamplifiedpolymorphicDNA ,RAPD)技术对来自陕西及青海的 7个地区的 1 3个落叶松—杨栅锈菌 (Melampsoralarici populinaKleb.)的分离物进行了基因组DNA多态性分析。 1 3个 1 0 -核苷酸随机引物 (Operon公司 )对 1 3个菌株共扩增出 81条RAPD带 ,其中 69个DNA片断呈现多态性 ,占总扩增片断的 85.2 %。供试菌株的相似系数在 0 .60 8～ 1 .0 0 0之间 ,各菌株之间的差异在 0～3 3 1 %之间 ,并建立了聚类树状图。 1 3个菌株在相似性 76.1 %时被分为 4个类群 :I组包括秦岭宁陕火地塘C的 2个分离物 ,II组为火地塘B的 1个分离物 ;III组为青海西宁、互助 ,陕西太白宝太路、宝鸡天台山、周至厚畛子 (HZa)等 5个地区的 8个分离物 ;第IV组为陕西周至厚畛子的 2个菌株 (HZb、HZc)。并与各菌株对不同杨树致病性的聚类分析进行了比较 ,结果表明各菌株间的DNA多态性与致病性及寄主、地理来源等没有明显相关性。The genomic DNA polymorph on 13 isolator of \%Melampsora larici\|populina\% Kleb. from Shaanxi and Qinghai were analyzed using random amplified polymorphism DNA markers (RAPD). 81 RAPD bands were amplified with 13 decanucleotide random primers, 69 bands of them were polymorphic(85.2%).Similarity coefficient of isolates was 0.608～1.000 and difference among isolates was 0～33 1%. The tested 13 isolates of the group were divided into 4 clusters with the similarities and genetic distances among them. Two isolates from Huoditang C in Qingling were placed in one group(group I) and one isolate from Huoditang B in another group(group II). Six isolates from Xining, Huzhu in Qinghai and Taibeibaotailu, Tiantaishan, Houzhengzi(HZa) in Shaanxi were placed in the third group(group III). Last two isolates from Houzhengzi(HZa,HZb) were placed in the fourth group(group IV).However, the DNA polymorphic of isolations were not obviously related to the collecting places or hosts and pathogenicity.

关 键 词：杨树 落叶松-杨栅锈菌 RAPD 致病性 

分 类 号：S763.721.1[农业科学—森林保护学]