松材线虫病木快速检疫技术的研究  

A STUDY ON THE TECHNIQUES OF THE RAPID QUARANTINE DETECTION FOR DISEASED WOOD CAUSED BY PINE WOOD NEMATODE

作　　者：王玉嬿[1] 舒朝然[1] 李海燕[1] 郭志红[1] 

机构地区：[1]国家林业局森林病虫害防治总站,沈阳110034

出　　处：《林业科学》2000年第5期59-62,共4页Scientia Silvae Sinicae

基　　金：国家"九.五"科技攻关专题!"松材线虫病防治技术研究"的部分内容

摘　　要：根据松材线虫病死木的化学成分中酸性物质增加 ,pH值明显下降的规律 (尤纪雪等 ,1 994 ) ,本研究探讨了利用指示剂在松材线虫Bursaphelenchusxylophilus(SteinerBurher)Nickle病、健木钻木屑的乙醇提取液中的颜色反应来进行快速检疫检验的可行性。通过对我国江苏、安徽、浙江、广东 4个疫区的 5种松树的试验结果表明 :利用指示剂 1号进行快速检疫检验 ,其准确率黑松 96%;马尾松 89%;赤松 1 0 0 %;火炬松92 %。除湿地松 (江苏 )外 ,均已达到检疫检验所要求的准确程度。本方法可操作性强 ,颜色反应直观 ,准确率高 ,简便快速 ,整个操作过程不超过 1 5min,且多个样品可同时进行。The higher amount acid chemical composition in the pine wilt diseased wood caused by the pine wood nematode, \%Bursaphelenchus xylophilus\% resulted in a decline in pH\|value compared with that of the healthy wood. Accordingly, the color reaction was useful for rapid quarantine detection by using a suitable pH\|indicator to test the alcohol liquids extracted from the diseased and healthy wood crumb. Five pine species from Jiangsu, Anhui, Zhejiang and Guangdong Provinces were tested. The results showed that the quarantine detection accuracy by using pH\|indicator No.1 was 95% for \%Pinus thunbergii,\% 89% for \%P.massoniana,\%100% for \%P.densiflora,\%92% for \%P.teada.\% The method was available for the rapid quarantine detection of the pine species, but not for that of \%P.elliottii \%from Jiangsu Province. The color reaction was visual, accurate, simple, practical and rapid. The process was finished in 15 min. for one sample. Several samples could be detected at the same time.

关 键 词：松材线虫病 快速检疫 颜色反应 病木 木材检疫 

分 类 号：S763[农业科学—森林保护学]